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通过三色流式细胞术方法获得的绝对CD4计数，未使用血液学分析仪。

Absolute CD4 counts obtained by a three-color flow-cytometric method without the use of a hematology analyzer.

作者信息

Alexander T S

机构信息

Department of Pathology, Summa Health System, Akron, Ohio 44309, USA.

出版信息

Clin Diagn Lab Immunol. 1998 Mar;5(2):266-9. doi: 10.1128/CDLI.5.2.266-269.1998.

DOI:10.1128/CDLI.5.2.266-269.1998
PMID:9521157
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC121372/
Abstract

We evaluated the Ortho TRIO-Cytoronabsolute system for determining absolute CD4 counts. The CD4 counts in our blood specimens from 100 individuals ranged from 3 to 1,962; the percent CD4 ranged from 1.3 to 62.2, respectively. The TRIO system was biased toward lower absolute counts than a combination of flow cytometry and hematology but showed no bias in percent CD4 calculations.

摘要

我们评估了用于测定绝对CD4细胞计数的Ortho TRIO-Cytoronabsolute系统。我们从100名个体采集的血液标本中的CD4细胞计数范围为3至1962；CD4百分比分别为1.3至62.2。与流式细胞术和血液学联合检测相比，TRIO系统测得的绝对细胞计数偏低，但在CD4百分比计算方面无偏差。

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本文引用的文献

1
Evaluation of a method for counting absolute numbers of cells with a flow cytometer.流式细胞仪计数细胞绝对数量方法的评估
Clin Diagn Lab Immunol. 1997 May;4(3):309-13. doi: 10.1128/cdli.4.3.309-313.1997.
2
Use of CD45 fluorescence and side-scatter characteristics for gating lymphocytes when using the whole blood lysis procedure and flow cytometry.在使用全血裂解程序和流式细胞术时，利用CD45荧光和侧向散射特性对淋巴细胞进行设门。
Cytometry. 1996 Mar 15;26(1):16-21. doi: 10.1002/(SICI)1097-0320(19960315)26:1<16::AID-CYTO3>3.0.CO;2-E.
3
Standardization of absolute CD4+ lymphocyte counts across laboratories: an evaluation of the Ortho CytoronAbsolute flow cytometry system on normal donors.不同实验室间绝对CD4 + 淋巴细胞计数的标准化：对正常供体使用Ortho CytoronAbsolute流式细胞术系统的评估
Cytometry. 1995 Sep 15;22(3):200-10. doi: 10.1002/cyto.990220307.
4
Immunologic differentiation of absolute lymphocyte count with an integrated flow cytometric system: a new concept for absolute T cell subset determinations.
Cytometry. 1995 Mar 15;22(1):48-59. doi: 10.1002/cyto.990220110.