Connelly M C, Knight M, Giorgi J V, Kagan J, Landay A L, Parker J W, Page E, Spino C, Wilkening C, Mercolino T J
Immunocytometry Research and Development, Ortho Diagnostic Systems, Raritan, New Jersey 08869, USA.
Cytometry. 1995 Sep 15;22(3):200-10. doi: 10.1002/cyto.990220307.
The Ortho CytoronAbsolute is a flow cytometer designed to provide direct absolute counts of lymphocytes and their subsets from a single instrument. This study was designed to determine the performance of four geographically separated CytoronAbsolute instruments using 24-h-old, shipped, whole blood samples and to compare the results obtained on the CytoronAbsolute to those obtained using combinations of hematology instruments and other flow cytometers. The absolute count feature of the CytoronAbsolutes located at the four sites were cross calibrated and gave across-site coefficients of variation (CVs) of <4.0% for absolute count and 8.2% for absolute lymphocyte count. The calibration was stable for at least 2 months. Absolute lymphocyte counts and lymphocyte percentage immunophenotypes were determined on blood from 50 healthy human immunodeficiency virus (HIV)-seronegative donors. There were no significant site-to-site differences (each P > .05) in CD3+/CD4+ absolute lymphocyte counts determined on the CytoronAbsolute. In contrast, there was a significant site-to-site difference (P < .001) between sites 2 and 3 and sites 3 and 4 in the absolute CD3+/CD4+ lymphocyte counts determined via the conventional method of combining a flow cytometry-derived percentage with a hematology instrument-derived lymphocyte count. There was no significant difference (P = .388) in CD3+/CD4+ lymphocyte percent determinations between the CytoronAbsolute and the FACScan or Profile II flow cytometers used in this study. These results demonstrate that different operators can cross calibrate CytoronAbsolutes for absolute CD3+/CD4+ lymphocyte subset determinations, even over large geographic distances.
Ortho CytoronAbsolute是一款流式细胞仪，旨在通过一台仪器直接对淋巴细胞及其亚群进行绝对计数。本研究旨在使用运输24小时后的全血样本，测定四台地理位置分散的CytoronAbsolute仪器的性能，并将CytoronAbsolute获得的结果与血液学仪器和其他流式细胞仪组合获得的结果进行比较。位于四个地点的CytoronAbsolutes的绝对计数功能进行了交叉校准，绝对计数的跨地点变异系数（CVs）<4.0%，绝对淋巴细胞计数的变异系数为8.2%。校准至少稳定2个月。对50名健康人类免疫缺陷病毒（HIV）血清阴性供者的血液进行了绝对淋巴细胞计数和淋巴细胞百分比免疫表型测定。在CytoronAbsolute上测定的CD3+/CD4+绝对淋巴细胞计数在各地点之间无显著差异（各P>.05）。相比之下，通过将流式细胞术得出的百分比与血液学仪器得出的淋巴细胞计数相结合的传统方法测定的绝对CD3+/CD4+淋巴细胞计数在地点2和地点3以及地点3和地点4之间存在显著的地点间差异（P<.001）。本研究中使用的CytoronAbsolute与FACScan或Profile II流式细胞仪在CD3+/CD4+淋巴细胞百分比测定方面无显著差异（P =.388）。这些结果表明，即使跨越较大的地理距离，不同的操作人员也可以对CytoronAbsolutes进行交叉校准，以进行绝对CD3+/CD4+淋巴细胞亚群的测定。
