Savoia P, Novelli M, De Matteis A, Verrone A, Bernengo M G
1st Dermatologic Clinic, University of Turin, Torino, Italy.
J Cutan Pathol. 1998 Feb;25(2):89-94. doi: 10.1111/j.1600-0560.1998.tb01695.x.
Seven patients with chronic plaque psoriasis were treated with topical calcipotriol for 8 to 24 weeks; the lesions improved in 5 patients. Immunohistochemistry was performed on frozen sections, to evaluate the expression of adhesion molecules and extracellular matrix components before and after therapy. Changes in expression and topography of beta1 and beta4 integrins were found on psoriatic lesions before therapy and a reduction in the expression of tenascin was detected as well. Moreover, several activation markers such as ICAM-1, HLA-DR, CD26 were focally positive, with a diffuse cytoplasmic reactivity, in basal and suprabasal layers in untreated lesions. In the 5 patients in whom lesions regressed after topical calcipotriol treatment, we observed a histological normalization of the epidermis and the inflammatory infiltrate was reduced. Moreover, not only was there a normalization in the expression and topography of adhesion molecules, but also the integrin pattern observed after therapy was superimposable to that of normal skin.
7例慢性斑块状银屑病患者接受外用卡泊三醇治疗8至24周；5例患者皮损改善。对冰冻切片进行免疫组织化学检查，以评估治疗前后黏附分子和细胞外基质成分的表达。治疗前在银屑病皮损上发现β1和β4整合素的表达及分布改变，同时检测到肌腱蛋白表达减少。此外，在未经治疗的皮损基底层和基底上层，几种活化标志物如细胞间黏附分子-1（ICAM-1）、人类白细胞抗原-DR（HLA-DR）、CD26呈局灶性阳性，伴有弥漫性细胞质反应。在5例经外用卡泊三醇治疗后皮损消退的患者中，我们观察到表皮组织学恢复正常，炎症浸润减少。此外，不仅黏附分子的表达和分布恢复正常，而且治疗后观察到的整合素模式与正常皮肤的模式重叠。
