Dear P H, Bankier A T, Piper M B
Medical Research Council Laboratory of Molecular Biology, Cambridge, United Kingdom.
Genomics. 1998 Mar 1;48(2):232-41. doi: 10.1006/geno.1997.5140.
We have mapped 1001 novel sequence-tagged sites on human chromosome 14. The mean spacing between markers is approximately 90 kb, most markers are mapped with a resolution of better than 100 kb, and physical distances are determined. The map was produced using HAPPY mapping, a simple and widely applicable in vitro approach that is analogous to linkage or to radiation hybrid mapping, but that circumvents many of the difficulties and potential artifacts associated with these methods. We show also that the map serves as a robust scaffold for building physical maps using large-insert clones.
我们已在人类14号染色体上绘制了1001个新的序列标签位点。标记之间的平均间距约为90 kb，大多数标记的定位分辨率优于100 kb，并且确定了物理距离。该图谱是使用HAPPY作图法绘制的，这是一种简单且广泛适用的体外方法，类似于连锁作图或辐射杂种作图，但避免了与这些方法相关的许多困难和潜在假象。我们还表明，该图谱可作为使用大插入片段克隆构建物理图谱的强大支架。
