Rakhawy M T, Tarkhan A A, Zakaria A M
Acta Anat (Basel). 1976;95(1):130-41. doi: 10.1159/000144609.
(1) Cholinesterase activity was investigated in some human lymphatic organs (palatine tonsil, 'normal' spleen, 'bilharzial' spleen, thymus, lymph node and appendix) using GOMORI'S modification of KOELLE and FRIEDENWALD'S thiocholine iodide method, hydrolyzing acetylthiocholine iodide and butyrylthiocholine iodide. (a) Acetyl- and butyrylcholinesterases seemed to be different enzymes; but when they have the same pattern of activity, the latter generally offers a weaker reaction. (b) All the lymphatic follicles of the tonsil, those found in the cortex of the cervical lymph nodes as well as those present in the appendix, were stainable with both acetyl- and butyrylcholinesterase. (c) Acetylcholinesterase activity was not demonstrated in the Malpighian bodies of the 'normal' spleen, but the reaction was strongly present in the blood vessels (including the central arterioles) as well as in the capsule and the different components of the trabecular system. (d) In 'bilharzial' splenomegaly a relatively strong activity started to appear in the Malpighian corpuscles, manifested as a brownish precipitate in their centres. Also some patchy positive areas began to make their appearance in the tissue of the red pulp and had a particular arrangement around the Malpighian corpuscules, in such a way as to 'wall them off' from the tissue of the red pulp. (e) In the thymus no acetylcholinesterase activity was encountered, except in Hassal's corpuscles and in the trabeculae between the thymic lobules. (2) The data obtained in this work were discussed in relation to previous works in other laboratories and it seems that a species difference exists. (3) Cholinesterases may be present in the lymphatic tissue in order to get rid of some potentially toxic esters resulting from the necrobiotic phenomena accompanying the high mitotic activity found especially in the germinal centres of the lymphoid follicles. (4) There are many unanswered questions about the coexistence of the phosphatases and cholinesterases in the same places; their concomitant association in the lymphatic tissue may represent a special case within the framework of a more general mechanism.
(1) 采用戈莫里对科勒和弗里登瓦尔德硫代胆碱碘化物法的改进方法，通过水解碘化乙酰硫代胆碱和碘化丁酰硫代胆碱，对一些人体淋巴器官（腭扁桃体、“正常”脾脏、“血吸虫性”脾脏、胸腺、淋巴结和阑尾）中的胆碱酯酶活性进行了研究。(a) 乙酰胆碱酯酶和丁酰胆碱酯酶似乎是不同的酶；但当它们具有相同的活性模式时，后者通常反应较弱。(b) 扁桃体的所有淋巴滤泡、颈淋巴结皮质中的淋巴滤泡以及阑尾中的淋巴滤泡，均可被乙酰胆碱酯酶和丁酰胆碱酯酶染色。(c) “正常”脾脏的马氏体中未显示乙酰胆碱酯酶活性，但在血管（包括中央小动脉）以及包膜和小梁系统的不同组成部分中反应强烈。(d) 在“血吸虫性”脾肿大时，马氏体中开始出现相对较强的活性，表现为其中心的褐色沉淀。此外，在红髓组织中也开始出现一些散在的阳性区域，并且围绕马氏体有特殊的排列方式，从而将它们与红髓组织“分隔开”。(e) 在胸腺中，除了哈氏小体和胸腺小叶之间的小梁外，未发现乙酰胆碱酯酶活性。(2) 结合其他实验室先前的研究工作对本研究获得的数据进行了讨论，似乎存在种属差异。(3) 胆碱酯酶可能存在于淋巴组织中，以便清除一些由尤其在淋巴滤泡生发中心发现的高有丝分裂活性伴随的细胞坏死现象产生的潜在有毒酯类。(4) 关于磷酸酶和胆碱酯酶在同一部位共存存在许多未解决的问题；它们在淋巴组织中的同时存在可能代表了更一般机制框架内的一种特殊情况。
