Rejnö S
Acta Vet Scand. 1976;17(2):178-89. doi: 10.1186/BF03547926.
LDH is an intracellular enzyme, which when cells degenerate is released to the extracellular spaces and body fluids. Cells and organs in the mammalian body differ from each other with respect to their LDH isoenzyme patterns. These circumstances have led to the use of LDH isoenzyme determinations in laboratory diagnostic work. In the present investigation total LDH activity and LDH isoenzyme distribution in equine synovial fluid from healthy joints, joints with serous arthritis, osteochondrosis dissecans and arthrosis, were determined. The fluids from the diseased joints differed from normal synovial fluid with respect to total LDH activity, and the different joint diseases each seemed to give rise to a characteristic isoenzyme pattern. In order to examine possible sources of the increased LDH activity and altered isoenzyme patterns, blood plasma, red and white blood cells, synovial membrane and articular cartilage were also studied. It was found that LDH4 and LDH5 were present in high amounts in articular cartilage, and an increase in these isoenzymes was the most characteristic feature in synovial fluid from joints with arthrosis. The results were discussed in view of possible diagnostic value of isoenzyme determinations on synovial fluid.
乳酸脱氢酶（LDH）是一种细胞内酶，当细胞发生变性时，它会释放到细胞外间隙和体液中。哺乳动物体内的细胞和器官，其乳酸脱氢酶同工酶模式各不相同。这些情况使得乳酸脱氢酶同工酶测定在实验室诊断工作中得到应用。在本研究中，测定了来自健康关节、浆液性关节炎关节、剥脱性骨软骨炎关节和关节病关节的马滑液中的总乳酸脱氢酶活性及乳酸脱氢酶同工酶分布。患病关节的滑液在总乳酸脱氢酶活性方面与正常滑液不同，而且不同的关节疾病似乎各自产生一种特征性的同工酶模式。为了研究乳酸脱氢酶活性增加和同工酶模式改变的可能来源，还对血浆、红细胞和白细胞、滑膜及关节软骨进行了研究。结果发现，关节软骨中大量存在乳酸脱氢酶4和乳酸脱氢酶5，这些同工酶的增加是关节病关节滑液中最具特征性的特点。鉴于滑液同工酶测定的可能诊断价值，对结果进行了讨论。
