Allen M J, Hoffmann W E, Richardson D C, Breur G J
Department of Orthopedic Surgery, State University of New York, Syracuse 13210, USA.
Am J Vet Res. 1998 Mar;59(3):250-4.
To establish reference values for a panel of serum markers of bone turnover in dogs of various ages.
Dogs in 4 age groups (0 to 1 year; 1 to 2 years; 3 to 7 years; > 8 years).
Serum concentrations of the carboxyterminal propeptide of type-I procollagen (PICP) and the aminoterminal propeptide of type-I procollagen (PINP), both markers of type-I collagen synthesis (hence, bone formation), were measured by use of commercial human radioimmunoassay kits. Serum concentrations of the carboxyterminal cross-linked telopeptide of type-I collagen (ICTP), a marker for type-I collagen breakdown (hence, bone resorption), also were measured by use of a commercial human radioimmunoassay kit. Serum osteocalcin (OC) concentrations and alkaline phosphatase (ALP) isoenzyme activities were measured by use of techniques developed specifically for dogs.
As expected, the highest values for all of the markers were found in young dogs (< 12 months old). Concentrations of OC and ICTP decreased with age, and were lowest in dogs > 8 years old. Total ALP and bone-specific ALP activities initially decreased with age, then increased in dogs > 8 years old.
Serum markers of bone turnover may be useful diagnostic and prognostic tools for management of dogs with musculoskeletal disorders.
建立不同年龄犬骨转换血清标志物组的参考值。
4个年龄组的犬（0至1岁；1至2岁；3至7岁；>8岁）。
使用商业化的人放射免疫分析试剂盒测量I型前胶原羧基末端前肽（PICP）和I型前胶原氨基末端前肽（PINP）的血清浓度，这两种标志物均为I型胶原合成（即骨形成）的标志物。还使用商业化的人放射免疫分析试剂盒测量I型胶原羧基末端交联端肽（ICTP）的血清浓度，ICTP是I型胶原降解（即骨吸收）的标志物。血清骨钙素（OC）浓度和碱性磷酸酶（ALP）同工酶活性采用专门为犬开发的技术进行测量。
正如预期的那样，所有标志物的最高值在幼犬（<12月龄）中被发现。OC和ICTP的浓度随年龄增长而降低，在>8岁的犬中最低。总ALP和骨特异性ALP活性最初随年龄增长而降低，然后在>8岁的犬中升高。
骨转换血清标志物可能是用于管理患有肌肉骨骼疾病犬的有用诊断和预后工具。
