Lester G D, Merritt A M, Neuwirth L, Vetro-Widenhouse T, Steible C, Rice B, Mauragis D F, Madison J B
Department of Large Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, Gainesville 32610-0125, USA.
Am J Vet Res. 1998 Mar;59(3):313-9.
To determine normal cecal emptying curves for liquid- and solid-phase radiolabeled markers and to further define myoelectric patterns of the ileum, cecum, and colon in healthy ponies.
6 adult ponies.
A cecal cannula and 12 bipolar Ag-AgCl recording electrodes were sutured to the ileum, cecum, and right ventral colon of the ponies. Radioisotopes, indium 111-labeled diethyltriaminepentaacetic acid (111In-DTPA) and technetium 99m (99mTc)-labeled sulfur colloid bound to egg albumen, were introduced through the cannula directly into the cecal body. Movement of these markers from the cecum was monitored by a gamma camera, and power exponential clearance curves were generated. Myoelectric data were collected before and after i.v. administration of isotonic saline (0.9% NaCl) solution, and were analyzed for spike burst (SB) rate, relative activity index, and mean burst duration. Myoelectric complexes were identified from observation of chart recordings or compressed, digitized data.
Clearance curves were generated for liquid (111In-DTPA)- and solid (99mTc)-phase markers. Marker types were not different with respect to lag phase, but liquid markers emptied at a slightly faster rate than did solids. Baseline values were calculated after saline solution administration for each of the myoelectric variables investigated. A relation between ileal, cecal, and colonic myoelectric activity was identified. Activity consistent with the previously described colonic migrating myoelectric complex in the pelvic flexure was identified in the right ventral colon.
Baseline data on normal cecal emptying was obtained; this technique could be used to evaluate the effect of postulated motility-modifying treatments used in equine practice.
确定液相和固相放射性标记物的正常盲肠排空曲线，并进一步明确健康小马回肠、盲肠和结肠的肌电模式。
6匹成年小马。
将一根盲肠插管和12个双极银-氯化银记录电极缝合到小马的回肠、盲肠和右腹侧结肠。通过插管将放射性同位素，即铟111标记的二乙三胺五乙酸（111In-DTPA）和与蛋清结合的锝99m（99mTc）标记的硫胶体，直接注入盲肠体部。用γ相机监测这些标记物从盲肠的移动情况，并生成幂指数清除曲线。在静脉注射等渗盐水（0.9%氯化钠）溶液前后收集肌电数据，并分析其峰暴发放（SB）率、相对活动指数和平均暴发持续时间。通过观察图表记录或压缩的数字化数据来识别肌电复合体。
生成了液相（111In-DTPA）和固相（99mTc）标记物的清除曲线。标记物类型在延迟期方面没有差异，但液相标记物的排空速度略快于固相标记物。对每个研究的肌电变量在给予盐水溶液后计算基线值。确定了回肠、盲肠和结肠肌电活动之间的关系。在右腹侧结肠中发现了与先前描述的盆腔弯曲处结肠移行性肌电复合体一致的活动。
获得了正常盲肠排空的基线数据；该技术可用于评估马匹实践中假定的运动调节治疗的效果。
