Ormerod A D, Weller R, Copeland P, Benjamin N, Ralston S H, Grabowksi P, Herriot R
Department of Dermatology, University of Aberdeen, UK.
Arch Dermatol Res. 1998 Jan-Feb;290(1-2):3-8. doi: 10.1007/s004030050268.
Biopsies from psoriasis lesions and clinically uninvolved skin of eight patients and five normal subjects were studied by immunocytochemistry with computerized image analysis for the presence of endothelial, neuronal and inducible isoforms of nitric oxide synthase. Endothelial nitric oxide synthase was expressed in the endothelium and weakly in some keratinoctyes. Its expression was not significantly different in psoriasis. Inducible nitric oxide synthase, however, was absent from normal skin but was significantly upregulated in psoriatic lesional skin, focally in keratinocytes but to the greatest extent in the papillary dermis and to a lesser extent in clinically uninvolved psoriatic skin. Inducible nitric oxide synthase staining was greatest in the more severe lesions and correlated with the inflammatory infiltrate (CD3-positive cells) and with keratinocyte proliferation (Ki-67-positive cells). In normal skin, neuronal nitric oxide synthase was expressed only in keratinocytes in the granular layer and eccrine sweat glands. However, in psoriasis and clinically uninvolved skin the neuronal form was present through all levels of the epidermis. Direct measurement of nitric oxide production from the skin surface revealed a tenfold increase in the lesions of 16 psoriatic patients compared with their nonlesional skin, and this nitric oxide production was inhibited by topical betamethasone.
对8例患者和5名正常受试者的银屑病皮损及临床未受累皮肤进行活检，采用免疫细胞化学结合计算机图像分析技术，检测内皮型、神经型和诱导型一氧化氮合酶的表达情况。内皮型一氧化氮合酶在内皮细胞中表达，在一些角质形成细胞中表达较弱。其在银屑病中的表达无显著差异。然而，诱导型一氧化氮合酶在正常皮肤中不存在，但在银屑病皮损皮肤中显著上调，在角质形成细胞中呈局灶性表达，但在乳头真皮中表达程度最高，在临床未受累的银屑病皮肤中表达程度较低。诱导型一氧化氮合酶染色在较严重的皮损中最为明显，且与炎症浸润（CD3阳性细胞）和角质形成细胞增殖（Ki-67阳性细胞）相关。在正常皮肤中，神经型一氧化氮合酶仅在颗粒层角质形成细胞和小汗腺中表达。然而，在银屑病及临床未受累皮肤中，神经型一氧化氮合酶在表皮各层均有表达。对16例银屑病患者皮肤表面一氧化氮生成的直接测量显示，与非皮损皮肤相比，皮损处一氧化氮生成增加了10倍，且局部应用倍他米松可抑制这种一氧化氮生成。
