Department of Pharmaceutical Sciences, Rashtrasant Tukadoji Maharaj Nagpur University, Nagpur, Maharashtra, India.
Department of Pharmaceutical Sciences, Rashtrasant Tukadoji Maharaj Nagpur University, Nagpur, Maharashtra, India; Department of Chemistry, Western Kentucky University, Bowling Green, KY, United States.
Indian J Pharmacol. 2021 Mar-Apr;53(2):125-131. doi: 10.4103/ijp.IJP_506_19.
The present investigation was undertaken to develop a psoriatic-like skin inflammation rat model using imiquimod (IMQ) as an inducing agent.
The hairs of the back dorsal portion of the Wistar rats were removed and 80, 100, and 120 mg of IMQ cream (5% w/w) for 10 consecutive days was applied to different groups of rats. Further, psoriasis area severity index was used for calculating the psoriatic score, which included scoring of erythema, scaling, and thickening. Various biochemical parameters, pro-inflammatory cytokines, vascular endothelial growth factor (VEGF), and histopathological examination were also performed.
The results demonstrated signs of erythema, scaling, and thickening on group applied with 120 mg and 100 mg of IMQ along with ear thickening. Biochemical evaluation revealed a significant increase in the granulation tissue weight followed by significant decrease in the levels of collagen and hexosamine. The antioxidant parameters superoxide dismutase and catalase were found to decline, while nitric oxide and lipid peroxidation were significantly elevated in skin lesions, also supported by increased pro-inflammatory cytokines expression, i.e., interleukin (IL)-1 β, IL-6, IL-17, tumor necrosis factor-α, and VEGF. Histopathological studies revealed a disturbed natural structure along with increased epidermal proliferation, abnormal differentiation with increased number of keratinocytes in the psoriatic skin tissue.
From the overall study, we have successfully developed a psoriatic-like skin inflammation rat model for the first time on Wistar strain using IMQ as an inducing agent.
本研究旨在使用咪喹莫特（IMQ）作为诱导剂，建立一种银屑病样皮肤炎症大鼠模型。
去除 Wistar 大鼠背部背侧的毛发，将 80、100 和 120mg 的 IMQ 乳膏（5%w/w）连续 10 天应用于不同组别的大鼠。此外，还使用银屑病面积严重程度指数（PASI）计算银屑病评分，包括红斑、鳞屑和增厚评分。还进行了各种生化参数、促炎细胞因子、血管内皮生长因子（VEGF）和组织病理学检查。
结果显示，应用 120mg 和 100mg IMQ 的组出现红斑、鳞屑和增厚，同时耳厚。生化评估显示肉芽组织重量显著增加，随后胶原蛋白和己糖胺水平显著降低。抗氧化参数超氧化物歧化酶和过氧化氢酶水平下降，而皮肤损伤中的一氧化氮和脂质过氧化水平显著升高，同时促炎细胞因子表达增加，即白细胞介素（IL）-1β、IL-6、IL-17、肿瘤坏死因子-α和 VEGF。组织病理学研究显示，银屑病皮肤组织中天然结构受到干扰，表皮增殖增加，角化细胞异常分化，数量增加。
综上所述，我们首次成功地使用 IMQ 作为诱导剂，在 Wistar 大鼠上建立了一种银屑病样皮肤炎症大鼠模型。
