果蝇中支架相关区域的体内分析：一种合成的高亲和力支架相关区域结合蛋白抑制位置效应斑驳。
In vivo analysis of scaffold-associated regions in Drosophila: a synthetic high-affinity SAR binding protein suppresses position effect variegation.
作者信息
Girard F, Bello B, Laemmli U K, Gehring W J
机构信息
Department of Cell Biology, Biozentrum, University of Basel, Klingelbergstrasse 70, 4056 Basel, Switzerland.
出版信息
EMBO J. 1998 Apr 1;17(7):2079-85. doi: 10.1093/emboj/17.7.2079.
Abstract
Scaffold-associated regions (SARs) were studied in Drosophila melanogaster by expressing a synthetic, high-affinity SAR-binding protein called MATH (multi-AT-hook), which consists of reiterated AT-hook peptide motifs; each motif is known to recognize a wide variety of short AT-rich sequences. MATH proteins were expressed specifically in the larval eye imaginal discs by means of the tetracycline-regulated transactivation system and tested for their effect on position effect variegation (PEV). MATH20, a highly potent SAR ligand consisting of 20 AT-hooks, was found to suppress whitemottled 4 variegation. This suppression required MATH20 expression at an early larval developmental stage. Our data suggest an involvement of the high AT-rich SARs in higher order chromatin structure and gene expression.
摘要
通过表达一种名为MATH（多AT钩）的合成高亲和力支架相关区域（SAR）结合蛋白，在黑腹果蝇中研究了SAR。MATH由重复的AT钩肽基序组成；已知每个基序可识别多种短的富含AT的序列。借助四环素调控的反式激活系统，MATH蛋白在幼虫眼成虫盘中特异性表达，并测试其对位置效应斑驳（PEV）的影响。发现由20个AT钩组成的高效SAR配体MATH20可抑制白色斑驳4的斑驳现象。这种抑制需要在幼虫发育早期表达MATH20。我们的数据表明，富含AT的高SAR参与了高阶染色质结构和基因表达。
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