Odenheimer Jens, Kreth Gregor, Heermann Dieter W
Institut für Theoretische Physik, Universität Heidelberg, Philosophenweg 19, D-69120 Heidelberg, Germany.
J Biol Phys. 2005 Dec;31(3-4):351-63. doi: 10.1007/s10867-005-7286-3.
In the present study a model for the compactification of the 30 nm chromatin fibre into higher order structures is suggested. The idea is that basically every condensing agent (HMG/SAR, HP1, cohesin, condensin, DNA-DNA interaction …) can be modeled as an effective attractive potential of specific chain segments. This way the formation of individual 1 Mbp sized rosettes from a linear chain could be observed. We analyse how the size of these rosettes depends on the number of attractive segments and on the segment length. It turns out that 8-20 attractive segments per 1 Mbp domain produces rosettes of 300-800 nm in diameter. Furthermore, our results show that the size of the rosettes is relatively insensitive to the segment length.
在本研究中，我们提出了一个将30纳米染色质纤维压缩成更高阶结构的模型。其核心观点是，基本上每种凝聚剂（HMG/SAR、HP1、黏连蛋白、凝聚素、DNA-DNA相互作用……）都可以被建模为特定链段的有效吸引势。通过这种方式，可以观察到从线性链形成单个1兆碱基大小的玫瑰花结。我们分析了这些玫瑰花结的大小如何依赖于吸引段的数量和段长度。结果表明，每1兆碱基结构域有8 - 20个吸引段时会产生直径为300 - 800纳米的玫瑰花结。此外，我们的结果表明，玫瑰花结的大小对段长度相对不敏感。
