Singleton T L, Wilcox E
The National Institute on Deafness, Other Communication Disorders, Laboratory of Molecular Genetics, National Institutes of Health, Bethesda, MD 20850, USA.
Gene. 1998 Mar 16;209(1-2):131-8. doi: 10.1016/s0378-1119(98)00025-0.
The full-length mouse RNA polymerase II (pol II) largest subunit (RPB1) gene was used to replace 5070 bp of the yeast Saccharomyces cerevisiae RPB1 gene via homologous recombination and gene replacement in vivo. Transcription of the mouse RPB1 gene using the yeast promoter in the haploid state was confirmed by Northern analysis. This strain of yeast is viable, indicating that mouse RPB1 is able to interact functionally with the other yeast RNA pol II subunits in vivo.
通过体内同源重组和基因置换，使用全长小鼠RNA聚合酶II（pol II）最大亚基（RPB1）基因替换酿酒酵母RPB1基因的5070 bp。通过Northern分析证实了单倍体状态下使用酵母启动子的小鼠RPB1基因的转录。这种酵母菌株是有活力的，表明小鼠RPB1能够在体内与其他酵母RNA pol II亚基进行功能相互作用。
