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一个高度保守的哺乳动物对应物对酵母必需RNA聚合酶亚基的功能替代。

Functional substitution of an essential yeast RNA polymerase subunit by a highly conserved mammalian counterpart.

作者信息

McKune K, Woychik N A

机构信息

Roche Institute of Molecular Biology, Nutley, New Jersey 07110.

出版信息

Mol Cell Biol. 1994 Jun;14(6):4155-9. doi: 10.1128/mcb.14.6.4155-4159.1994.

DOI:10.1128/mcb.14.6.4155-4159.1994
PMID:8196653
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC358781/
Abstract

We isolated the cDNA encoding the homolog of the Saccharomyces cerevisiae nuclear RNA polymerase common subunit RPB6 from hamster CHO cells. Alignment of yeast RPB6 with its mammalian counterpart revealed that the subunits have nearly identical carboxy-terminal halves and a short acidic region at the amino terminus. Remarkably, the length and amino acid sequence of the hamster RPB6 are identical to those of the human RPB6 subunit. The conservation in sequence from lower to higher eukaryotes also reflects conservation of function in vivo, since hamster RPB6 supports normal wild-type yeast cell growth in the absence of the essential gene encoding RPB6.

摘要

我们从仓鼠CHO细胞中分离出了编码酿酒酵母核RNA聚合酶共同亚基RPB6同源物的cDNA。酵母RPB6与其哺乳动物对应物的比对显示，这些亚基具有几乎相同的羧基末端半部以及氨基末端的一个短酸性区域。值得注意的是，仓鼠RPB6的长度和氨基酸序列与人RPB6亚基的长度和氨基酸序列相同。从低等真核生物到高等真核生物的序列保守性也反映了体内功能的保守性，因为在缺乏编码RPB6的必需基因的情况下，仓鼠RPB6支持正常的野生型酵母细胞生长。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3dd4/358781/cfcaa428f699/molcellb00006-0640-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3dd4/358781/cfcaa428f699/molcellb00006-0640-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3dd4/358781/cfcaa428f699/molcellb00006-0640-a.jpg

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本文引用的文献

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Gene Expr. 1993;3(1):77-82.
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RNA polymerase II subunit RPB10 is essential for yeast cell viability.
J Biol Chem. 1993 Jun 5;268(16):12230.
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RPB7, one of two dissociable subunits of yeast RNA polymerase II, is essential for cell viability.RPB7是酵母RNA聚合酶II的两个可解离亚基之一，对细胞生存能力至关重要。
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Comparison of the RNA polymerase III transcription machinery in Schizosaccharomyces pombe, Saccharomyces cerevisiae and human.粟酒裂殖酵母、酿酒酵母和人类中RNA聚合酶III转录机制的比较。
Nucleic Acids Res. 2001 Jul 1;29(13):2675-90. doi: 10.1093/nar/29.13.2675.
5
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RNA polymerase subunit RPB5 plays a role in transcriptional activation.RNA聚合酶亚基RPB5在转录激活中发挥作用。
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