Azuma Y, Yamagishi M, Ueshima R, Ishihama A
Department of Molecular Genetics, National Institute of Genetics, Shizuoka, Japan.
Nucleic Acids Res. 1991 Feb 11;19(3):461-8. doi: 10.1093/nar/19.3.461.
The gene, rpb1, encoding the largest subunit of RNA polymerase II has been cloned from Schizosaccharomyces pombe using the corresponding gene, RPB1, of Saccharomyces cerevisiae as a cross-hybridization probe. We have determined the complete sequence of this gene, and parts of PCR-amplified rpb1 cDNA. The predicted coding sequence, interrupted by six introns, encodes a polypeptide of 1,752 amino acid residues in length with a molecular weight of 194 kilodaltons. This polypeptide contains eight conserved structural domains characteristic of the largest subunit of RNA polymerases from other eukaryotes and, in addition, 29 repetitions of the C-terminal heptapeptide found in all the eukaryotic RNA polymerase II largest subunits so far examined.
利用酿酒酵母的相应基因RPB1作为交叉杂交探针，从粟酒裂殖酵母中克隆出了编码RNA聚合酶II最大亚基的基因rpb1。我们已经确定了该基因的完整序列以及PCR扩增的rpb1 cDNA的部分序列。预测的编码序列被6个内含子打断，编码一个长度为1752个氨基酸残基、分子量为194千道尔顿的多肽。该多肽包含其他真核生物RNA聚合酶最大亚基特有的8个保守结构域，此外，在迄今为止检测的所有真核生物RNA聚合酶II最大亚基中都发现了29个C末端七肽重复序列。
