Cashmore G C, Few J D, Fowles C H
Ann Clin Biochem. 1976 May;13(3):371-8. doi: 10.1177/000456327601300122.
The method investigated used (3H) cortisol as the ligand, diluted human plasma as the binding protein, and florisil to separate free and bound cortisol. The precision and specificity were found to be adequate for routine use. The effects of such factors as delay in separation of plasma, storage of plasma, provenance of the binding protein, venepuncture, and venous occlusion have been studied. The extent and significance of short term, inter-daily, and longer term variation in plasma cortisol level are discussed.
所研究的方法使用（3H）皮质醇作为配体，稀释的人血浆作为结合蛋白，并用弗罗里硅土分离游离和结合的皮质醇。结果发现该方法的精密度和特异性足以用于常规检测。已研究了诸如血浆分离延迟、血浆储存、结合蛋白来源、静脉穿刺和静脉阻塞等因素的影响。讨论了血浆皮质醇水平短期、日间和长期变化的程度及意义。
