马疱疹病毒4型糖蛋白I和E基因的核苷酸序列

Nucleotide sequences of glycoprotein I and E genes of equine herpesvirus type 4.

作者信息

Damiani A M, Matsumura T, Yokoyama N, Maeda K, Miyazawa T, Kai C, Mikami T

机构信息

Department of Veterinary Microbiology, Faculty of Agriculture, University of Tokyo, Japan.

出版信息

J Vet Med Sci. 1998 Feb;60(2):219-25. doi: 10.1292/jvms.60.219.

DOI:10.1292/jvms.60.219

PMID:9524947

Abstract

The nucleotide sequences of the glycoprotein I (gI) and E (gE) genes of equine herpesvirus type 4 (EHV-4) strain TH20 were determined. The predicted region encoding the EHV-4 gI gene is 1,263 nucleotides, corresponding to a polypeptide of 420 amino acids in length. The predicted region encoding the EHV-4 gE gene is 1,647 nucleotides, corresponding to a polypeptide of 548 amino acids in length. The EHV-4 gI and gE genes show 74% and 85% identity at the amino acid level with those of equine herpesvirus type 1 (EHV-1), respectively. Furthermore, we have found an open reading frame homologous to the EHV-1 gene 75, which overlaps in part with the 3' end of EHV-4 gE gene. These sequence data will be useful for development of a modified live vaccine against equine herpesvirus type 1 and 4 infections.

摘要

测定了马疱疹病毒4型（EHV-4）TH20株糖蛋白I（gI）和E（gE）基因的核苷酸序列。预测的编码EHV-4 gI基因的区域为1263个核苷酸，对应于一个长度为420个氨基酸的多肽。预测的编码EHV-4 gE基因的区域为1647个核苷酸，对应于一个长度为548个氨基酸的多肽。EHV-4 gI和gE基因在氨基酸水平上与马疱疹病毒1型（EHV-1）的相应基因分别具有74%和85%的同一性。此外，我们发现了一个与EHV-1基因75同源的开放阅读框，它与EHV-4 gE基因的3'端部分重叠。这些序列数据将有助于开发针对马疱疹病毒1型和4型感染的改良活疫苗。