Cavaco-Silva P, Taveira N C, Rosado L, Lourenço M H, Moniz-Pereira J, Douglas N W, Daniels R S, Santos-Ferreira M O
Departamento de Microbiologia, Faculdade de Farmácia, Universidade de Lisboa, Lisbon, Portugal.
J Virol. 1998 Apr;72(4):3418-22. doi: 10.1128/JVI.72.4.3418-3422.1998.
To demonstrate that human immunodeficiency virus type 2 (HIV-2) mother-to-child transmission exists, HIV-2 isolates were obtained from both an asymptomatic mother (HIV-2 strain ARM), and her child (HIV-2 strain SAR), who had a diagnosis of AIDS. To determine their biological phenotype, primary isolates were used to infect various primary mononuclear cells and cell lines. HIV-2 ARM replicates in primary cells and Jurkat-tat, while HIV-2 SAR infects these cells plus SupT1, which led us to classify HIV-2 ARM as a slow/low virus and HIV-2 SAR as having an intermediate (slow/low-3) phenotype. Molecular analysis of the env region corresponding to gp125 was performed. Viral DNA was cloned, sequenced, and used to construct phylogenetic trees. The DNA sequence analysis demonstrated an overall nucleotide diversity of 7.6%. The results present evidence that the child's strain is more virulent than the mother's strain, which is in agreement with the immunodeficiency of the child. The phylogenetic trees that were constructed demonstrate that the two isolates cluster together, being closer to each other than to any other isolate described until now.
为证明存在人类免疫缺陷病毒2型（HIV-2）母婴传播，从一名无症状母亲（HIV-2毒株ARM）及其被诊断患有艾滋病的孩子（HIV-2毒株SAR）身上获取了HIV-2分离株。为确定它们的生物学表型，使用原始分离株感染各种原代单核细胞和细胞系。HIV-2 ARM在原代细胞和Jurkat-tat细胞中复制，而HIV-2 SAR可感染这些细胞以及SupT1细胞，这使我们将HIV-2 ARM归类为慢/低病毒，将HIV-2 SAR归类为具有中间型（慢/低-3）表型。对与gp125对应的env区域进行了分子分析。对病毒DNA进行克隆、测序并用于构建系统发育树。DNA序列分析显示总体核苷酸多样性为7.6%。结果表明，孩子的毒株比母亲的毒株毒性更强，这与孩子的免疫缺陷情况相符。构建的系统发育树表明，这两个分离株聚集在一起，彼此之间的关系比迄今描述的任何其他分离株都更密切。
