Goddemeier M L, Wulff D, Feix G
University of Freiburg, Institute of Biology III, Germany.
Plant Mol Biol. 1998 Mar;36(5):799-802. doi: 10.1023/a:1005998804622.
The isolation and characterization of a cDNA clone from Zea mays coding for a novel glycine-rich protein (GRP) is described. The corresponding 1.4 kb mRNA accumulates exclusively in roots (primary, lateral seminal and crown roots) of young maize seedlings, following developmentally specific patterns. In agreement with previously described GRPs from other plant species the derived protein sequence exhibits a hydrophobic domain at the N-terminal region followed by repeated glycine-rich motifs. Genomic Southern analysis indicates that the zmGRP3 gene is present in the maize genome as one or two copies or at a low copy number.
本文描述了从玉米中分离并鉴定一个编码新型富含甘氨酸蛋白(GRP)的cDNA克隆。相应的1.4 kb mRNA仅在玉米幼苗的根(主根、侧胚根和冠根)中按照发育特异性模式积累。与先前描述的其他植物物种的GRP一致,推导的蛋白质序列在N端区域显示出一个疏水结构域,随后是重复的富含甘氨酸基序。基因组Southern分析表明,zmGRP3基因在玉米基因组中以一个或两个拷贝存在,或者拷贝数较低。