Tao T Y, Ouellet T, Dadej K, Miller S S, Johnson D A, Singh J
Eastern Cereal and Oilseed Research Centre, Agriculture and Agri-Food Canada, K.W. Neatby Bldg., Room 2091, Ottawa, Ontario, Canada.
Plant Cell Rep. 2006 Aug;25(8):848-58. doi: 10.1007/s00299-006-0128-3. Epub 2006 Mar 10.
The isolation, characterization and regulation of expression of a maize silk-specific gene is described. zmgrp5 (Zea mays glycine-rich protein 5) encodes a 187 amino acid glycine-rich protein that displays developmentally regulated silk-specific expression. Northern, Western, in situ mRNA hybridization and transient gene expression analyses indicate that zmgrp5 is expressed in silk hair and in cells of the vascular bundle and pollen tube transmitting tissue elements. The protein is secreted into the extracellular matrix and is localized in the cell wall fraction mainly through interactions mediated by covalent disulphide bridges. Taken together, these results suggest that the protein may play a role in maintaining silk structure during development. This is the first documented isolation of a stigma-specific gene from maize, an important agronomic member of the Poaceae family.
本文描述了一个玉米花丝特异性基因的分离、特性鉴定及表达调控。zmgrp5（玉米富含甘氨酸蛋白5）编码一种含187个氨基酸的富含甘氨酸蛋白，其表达呈现出发育调控的花丝特异性。Northern杂交、Western杂交、原位mRNA杂交及瞬时基因表达分析表明，zmgrp5在丝状毛、维管束细胞以及花粉管传递组织细胞中表达。该蛋白分泌到细胞外基质中，主要通过共价二硫键介导的相互作用定位于细胞壁部分。综合这些结果表明，该蛋白可能在发育过程中维持花丝结构方面发挥作用。这是首次从玉米（禾本科重要的农艺作物）中分离出柱头特异性基因的文献报道。
