Miller J S, Wesley S V, Naidu R A, Reddy D V, Mayo M A
Scottish Crop Research Institute, Invergowrie, Dundee, U.K.
Arch Virol. 1996;141(12):2301-12. doi: 10.1007/BF01718632.
The nucleotide sequence of RNA-1 of an isolate of the H serotype of Indian peanut clump virus (IPCV-H) was shown to comprise 5,841 nucleotides. The RNA contains three open reading frames (ORF) which are between nucleotides 133 and 3,522, nucleotides 3,526 and 5,103 (assuming expression by suppression of the ORF 1 termination codon) and nucleotides 5,168 and 5,539. The encoded polypeptides have M(r), of 129,687 (p130), 60,188 (p60) and 14,281 (p14). ORF 2 is thought to be expressed by suppression of the termination codon of ORF 1 to produce a M(r) 189,975 product (p190). p130 contains sequences characteristic of proteins with methyl transferase and NTP-binding properties and p190 contains these and sequences characteristic of RNA-dependent RNA polymerases. The nucleotide sequence of IPCV RNA-1 is similar to that of peanut clump virus (PCV) and corresponding encoded polypeptides are 88% (p130), 95% p60 and 75% (p14) identical. The sequences of the translation products are also similar to those of soil-borne wheat mosaic virus and barley stripe mosaic virus. Oligonucleotide primers, designed on the basis of the sequences of RNA-1 of IPCV and PCV, were effective in reverse transcription-PCR amplification of these RNAs and that of IPCV isolates of the serologically distinct L and T serotypes.
印度花生丛簇病毒H血清型（IPCV-H）一个分离株的RNA-1核苷酸序列显示由5841个核苷酸组成。该RNA包含三个开放阅读框（ORF），分别位于核苷酸133至3522、核苷酸3526至5103（假设通过抑制ORF 1终止密码子进行表达）以及核苷酸5168至5539之间。所编码的多肽的相对分子质量分别为129687（p130）、60188（p60）和14281（p14）。ORF 2被认为是通过抑制ORF 1的终止密码子来表达，从而产生一个相对分子质量为189975的产物（p190）。p130含有具有甲基转移酶和NTP结合特性的蛋白质的特征序列，p190含有这些序列以及RNA依赖性RNA聚合酶的特征序列。IPCV RNA-1的核苷酸序列与花生丛簇病毒（PCV）的相似，相应编码的多肽的同一性分别为88%（p130）、95%（p60）和75%（p14）。翻译产物的序列也与土传小麦花叶病毒和大麦条纹花叶病毒的序列相似。根据IPCV和PCV的RNA-1序列设计的寡核苷酸引物，在逆转录PCR扩增这些RNA以及血清学上不同的L和T血清型的IPCV分离株的RNA时是有效的。
