Lot H, Rubino L, Delecolle B, Jacquemond M, Turturo C, Russo M
INRA, Station de Pathologie Végétale, Montfavet, France.
Arch Virol. 1996;141(12):2375-86. doi: 10.1007/BF01718638.
White stripe is a disease affecting leek in France with which an isometric virus c. 30 nm in diameter is associated. The most evident symptom is the presence of white stripes on the leaves extending to the stem. Attempts to demonstrate transmission through the soil by sowing or transplanting leek in contaminated soil were unsuccessful. The virus was transmitted by sap inoculation to a narrow range of herbaceous hosts, all of which were infected only locally. Virus purification was from infected leek tissues, where it accumulated in large amounts, as demonstrated by ultrastructural observations. RNA was extracted from purified virus preparations and cDNA clones were prepared. The complete nucleotide sequence of the viral RNA was determined: The genome is 3,662 nucleotides long and contains five open reading frames (ORFs). The first (ORF 1) encodes a putative translation product of M(r) 23,803 (p24) and read through of its amber stop codon results in a protein of M(r) 82,625 (p83) (ORF 2). ORF 3 and ORF 4 encode two small polypeptides of M(r) 11,280 (p11) and M(r) 6,261 (p6), respectively. ORF 5 encodes the capsid protein of M(r) 27,460 (p27). The genome organization and sequence alignments with the corresponding products of necroviruses suggest that the virus isolated from leek is a new species in the genus Necrovirus, for which the name of leek white stripe virus (LWSV) is proposed.
白条病是一种影响法国韭菜的病害，与一种直径约30纳米的等轴病毒有关。最明显的症状是叶片上出现白色条纹并延伸至茎部。通过在受污染土壤中播种或移栽韭菜来证明土壤传播的尝试未成功。该病毒通过汁液接种可传播至少数几种草本寄主植物，但所有这些寄主植物仅局部感染。病毒从感染的韭菜组织中纯化得到，超微结构观察表明病毒在该组织中大量积累。从纯化的病毒制剂中提取RNA并制备cDNA克隆。测定了病毒RNA的完整核苷酸序列：基因组长度为3662个核苷酸，包含五个开放阅读框（ORF）。第一个（ORF 1）编码一个推定的分子量为23803的翻译产物（p24），其琥珀色终止密码子的通读产生一个分子量为82625的蛋白质（p83）（ORF 2）。ORF 3和ORF 4分别编码两个小多肽，分子量分别为11280（p11）和6261（p6）。ORF 5编码分子量为27460的衣壳蛋白（p27）。基因组结构以及与坏死病毒相应产物的序列比对表明，从韭菜中分离出的病毒是坏死病毒属中的一个新种，为此提议将其命名为韭菜白条病毒（LWSV）。
