Guilley H, Carrington J C, Balàzs E, Jonard G, Richards K, Morris T J
Nucleic Acids Res. 1985 Sep 25;13(18):6663-77. doi: 10.1093/nar/13.18.6663.
The complete nucleotide sequence of carnation mottle genomic RNA (4003 nucleotides) is presented. The sequence was determined for cloned cDNA copies of viral RNA containing over 99% of the sequence and was completed by direct sequence analysis of RNA and cDNA transcripts. The sequence contains two long open reading frames which together can account for observed translation products. One translation product would arise by suppression of an amber termination codon and the sequence raises the possibility that a second suppression event could also occur. Sequence homology exists between a portion of the carnation mottle virus sequence and that of putative RNA polymerases from other RNA viruses.
本文给出了香石竹斑驳病毒基因组RNA的完整核苷酸序列(4003个核苷酸)。该序列是通过对包含超过99%序列的病毒RNA的克隆cDNA拷贝进行测定,并通过对RNA和cDNA转录本的直接序列分析完成的。该序列包含两个长开放阅读框,它们共同可以解释观察到的翻译产物。一种翻译产物可能是通过抑制琥珀色终止密码子产生的,并且该序列增加了第二种抑制事件也可能发生的可能性。香石竹斑驳病毒序列的一部分与其他RNA病毒的推定RNA聚合酶的序列之间存在序列同源性。