Adachi J, Morita S, Yasuda H, Miwa A, Ueno Y, Asano M, Tatsuno Y
Department of Legal Medicine, Kobe University School of Medicine, Japan.
Clin Chim Acta. 1998 Jan 30;269(2):137-45. doi: 10.1016/s0009-8981(97)00190-3.
We investigated the nitric oxide profile in the plasma of ten healthy controls and 29 patients hurt by the Kobe Earthquake. The levels of nitrite (NO2-) and nitrate (NO3-) were measured simultaneously by high-performance liquid chromatography (HPLC) with UV detection using the Griess reaction after the reduction of nitrate to nitrite. Arginine consumed in food or diet-derived nitrite had no effect on the plasma nitrite and nitrate contents of the healthy volunteers. Plasma nitrate was elevated in the crush syndrome patients; whereas neither nitrite nor nitrate was increased in patients with normal renal function. This finding suggests increased nitric oxide synthesis, decreased excretion of nitric oxide in the crush syndrome or both.
我们对10名健康对照者和29名受神户地震伤害的患者的血浆一氧化氮情况进行了研究。采用高效液相色谱法（HPLC）并结合紫外检测，在将硝酸盐还原为亚硝酸盐后，利用格里斯反应同时测定亚硝酸盐（NO2-）和硝酸盐（NO3-）的水平。食物中摄入的精氨酸或饮食来源的亚硝酸盐对健康志愿者的血浆亚硝酸盐和硝酸盐含量没有影响。挤压综合征患者的血浆硝酸盐水平升高；而肾功能正常的患者亚硝酸盐和硝酸盐均未升高。这一发现表明，挤压综合征中一氧化氮合成增加、一氧化氮排泄减少或两者兼而有之。
