Goissis G, Yoshioka S A, Braile D M, Ramirez V D
Instituto de Química de São Carlos, Universidade de São Paulo, Brazil.
Artif Organs. 1998 Mar;22(3):210-4. doi: 10.1046/j.1525-1594.1998.06006.x.
This work describes the results of the controlled crosslinking of collagen matrices by glutaraldehyde based on a double protection strategy, glutaraldehyde acetals and lysine protonation due to the acidic conditions of acetal formation. Materials crosslinked by this approach were characterized by thermal stability comparable to those obtained by conventional procedures with mechanical properties expected for bioprosthesis manufacture and with a higher stability toward collagenase hydrolysis. The integrity of the microfibrillar structure was confirmed by optical and scanning electronic microscopy. The results indicate that the glutaraldehyde acetals procedure may be of potential use for the crosslinking of bovine pericardium used in the manufacture of bioprosthetic devices. Advantages may be related to the production of materials with homogeneous crosslinking distributions, associated with better definition in the nature of the chemical link that they introduce, due to a better distribution of glutaraldehyde within the tissue matrix before the crosslinking reaction is allowed to occur. As a result, materials with improved biological and mechanical properties are expected.
这项工作描述了基于双重保护策略（戊二醛缩醛以及由于缩醛形成的酸性条件导致的赖氨酸质子化），通过戊二醛对胶原蛋白基质进行可控交联的结果。用这种方法交联的材料具有与传统方法获得的材料相当的热稳定性，具有生物假体制造所需的机械性能，并且对胶原酶水解具有更高的稳定性。通过光学显微镜和扫描电子显微镜证实了微纤维结构的完整性。结果表明，戊二醛缩醛法可能潜在地用于制造生物假体装置中所用牛心包的交联。优点可能与产生具有均匀交联分布的材料有关，这与它们引入的化学连接性质的更好定义相关，这是因为在交联反应发生之前戊二醛在组织基质中的分布更好。因此，有望获得具有改善的生物学和机械性能的材料。
