Barberis I L, Pájaro M C, Godino S D, Aichino C
Departamento de Microbiología e Inmunología, Universidad Nacional de Río Cuarto, Córdoba, Argentina.
Enferm Infecc Microbiol Clin. 1997 Nov;15(9):473-6.
Pseudomonas aeruginosa with inhibitory capacity in vitro was studied on Gardnerella vaginalis strains.
Antimicrobial activity was demonstrated by inhibitory halos of bacterial growth on solid media by two methods: crossed streak and agar well diffusion. The inhibitory activity of this substance produced by P. aeruginosa was characterized as bacteriocin by: activity spectrum sensitivity proteolytic enzyme, chloroform, heat, pH, ultraviolet, irradiation effect and molecular weight.
Four strains of P. aeruginosa producers of bacteriocins were chosen for this study and contacted with 40 strains of G. vaginalis. The producing strain D inhibited 70% of these G. vaginalis strains. The strains B and C inhibited 55% and 52.5%, respectively. The 3 strains presented a wide rank of action but the strain A had effect on a few strains of G. vaginalis.
This work showed the inhibitory in vitro effect of bacteriocins of P. aeruginosa on strains of G. vaginalis. The results obtained suggest the probable topic application of bacteriocins as an alternative of conventional therapeutic on this infection biological control.
对体外具有抑制能力的铜绿假单胞菌针对阴道加德纳菌菌株进行了研究。
通过两种方法在固体培养基上细菌生长的抑制晕圈来证明抗菌活性：交叉划线法和琼脂扩散法。通过以下方面将铜绿假单胞菌产生的这种物质的抑制活性表征为细菌素：活性谱、敏感性、蛋白水解酶、氯仿、热、pH值、紫外线、辐射效应和分子量。
选择了四株产生细菌素的铜绿假单胞菌菌株并使其与40株阴道加德纳菌接触。产生菌株D抑制了70%的这些阴道加德纳菌菌株。菌株B和C分别抑制了55%和52.5%。这三株菌株呈现出广泛的作用范围，但菌株A仅对少数阴道加德纳菌菌株有作用。
这项工作显示了铜绿假单胞菌细菌素对阴道加德纳菌菌株的体外抑制作用。所获得的数据表明细菌素作为这种感染生物防治中传统治疗方法的替代物可能的局部应用。
