阴道嗜酸乳杆菌产生的抑制阴道加德纳菌的抗菌蛋白。
Antimicrobial protein produced by vaginal Lactobacillus acidophilus that inhibits Gardnerella vaginalis.
作者信息
Aroutcheva A A, Simoes J A, Faro S
机构信息
Department of Obstetrics and Gynecology, Rush-Presbyterian-St. Luke's Medical Center, Chicago, IL 60612, USA.
出版信息
Infect Dis Obstet Gynecol. 2001;9(1):33-9. doi: 10.1155/S1064744901000060.
Abstract
OBJECTIVE
To isolate bacteriocin from a vaginal strain of Lactobacillus acidophilus.
METHODS
L. acidophilus 160 was grown on two media. The first was MRS broth for 18 hours; the cells were harvested, washed, and placed into a chemically defined medium. The second medium resembled vaginal fluid minus protein. Bacteriocin was precipitated from both media using ammonium sulfate. The growth-inhibiting activity of bacteriocin was determined by a bioassay using nine different isolates of Gardnerella vaginalis.
RESULTS
MRS broth is not a suitable medium for extracting bacteriocin, because it binds with Tween 80. Bacteriocin was isolated, without contaminating constituents, from chemically defined medium and identified as a single band by electrophoresis. Bacteriocin has a molecular weight of 3.8 kDa. All nine isolates of Gardnerella were inhibited by the bacteriocin isolated from L. acidophilus 160.
CONCLUSIONS
Bacteriocin produced by L. acidophilus 160 was isolated from the chemically defined medium (starvation medium) in a partially pure form. L. acidophilus 160 bacteriocin inhibited growth of all nine isolates of Gardnerella vaginalis.
摘要
目的
从阴道嗜酸乳杆菌菌株中分离细菌素。
方法
嗜酸乳杆菌160在两种培养基上生长。第一种是MRS肉汤，培养18小时；收集细胞，洗涤后置于化学成分明确的培养基中。第二种培养基类似于不含蛋白质的阴道液。使用硫酸铵从两种培养基中沉淀细菌素。通过对九种不同阴道加德纳菌分离株进行生物测定来确定细菌素的生长抑制活性。
结果
MRS肉汤不是提取细菌素的合适培养基，因为它会与吐温80结合。从化学成分明确的培养基中分离出无污染成分的细菌素，并通过电泳鉴定为单一条带。细菌素的分子量为3.8 kDa。嗜酸乳杆菌160分离出的细菌素抑制了所有九种阴道加德纳菌分离株的生长。
结论
嗜酸乳杆菌160产生的细菌素以部分纯化的形式从化学成分明确的培养基（饥饿培养基）中分离出来。嗜酸乳杆菌160细菌素抑制了所有九种阴道加德纳菌分离株的生长。
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