Deretic D
Department of Ophthalmology and Anatomy, University of Michigan, Ann Arbor, USA.
Electrophoresis. 1997 Dec;18(14):2537-41. doi: 10.1002/elps.1150181408.
Polarized sorting of rhodopsin in retinal rod photoreceptors is mediated by post-Golgi vesicles that bud from the trans-Golgi network and fuse with the specialized domain of the plasma membrane in the rod inner segment. This domain surrounds the cilium that connects the inner segment and the rod outer segment to which mature rhodopsin is delivered. To dissect the sorting machinery that regulates budding, targeting, and fusion of rhodopsin carrier vesicles, their GTP-binding protein composition has been studied using multiple means including high-resolution two-dimensional gel electrophoresis and [32P]GTP overlays of renatured proteins. These studies indicate a succession on rhodopsin-bearing vesicles of rab6, rab11, rab3 and rab8, all members of the small GTP-binding protein family of the known regulators of membrane trafficking. In this review the role of rab proteins in post-Golgi trafficking of rhodopsin is discussed.
视紫红质在视网膜视杆光感受器中的极化分选由高尔基体后囊泡介导，这些囊泡从反式高尔基体网络出芽，并与视杆内段质膜的特化区域融合。该区域围绕着连接内段和视杆外段的纤毛，成熟的视紫红质被输送到视杆外段。为了剖析调节视紫红质载体囊泡出芽、靶向和融合的分选机制，人们使用了多种方法研究其GTP结合蛋白组成，包括高分辨率二维凝胶电泳和复性蛋白的[32P]GTP覆盖分析。这些研究表明，在携带视紫红质的囊泡上依次出现rab6、rab11、rab3和rab8，它们都是已知膜运输调节因子的小GTP结合蛋白家族的成员。在这篇综述中，我们讨论了rab蛋白在视紫红质高尔基体后运输中的作用。
