Deretic D
Department of Ophthalmology, University of Michigan, Ann Arbor 48105, USA.
Eye (Lond). 1998;12 ( Pt 3b):526-30. doi: 10.1038/eye.1998.141.
Rod outer segment renewal in retinal rod photoreceptors is mediated by polarised sorting of rhodopsin, and its associated proteins and lipids, on post-Golgi vesicles that bud from the trans-Golgi network and fuse with the specialised domain of the plasma membrane in the rod inner segment. This domain surrounds the cilium that connects the inner segment and the rod outer segment to which mature rhodopsin is delivered. The intracellular sorting machinery that regulates budding, targeting and fusion of rhodopsin carrier vesicles has been studied using multiple means including a newly developed cell-free assay that reconstitutes vesicle budding. These studies have revealed an essential role for small GTP-binding protein rab6, as well as the carboxyl-terminal domain of rhodopsin, in the formation of post-Golgi vesicles. In this report their role in post-Golgi trafficking of rhodopsin and the maintenance of photoreceptor cell polarity and health is discussed.
视网膜视杆光感受器中的视杆外段更新是由视紫红质及其相关蛋白质和脂质在高尔基体后囊泡上的极化分选介导的，这些囊泡从反式高尔基体网络出芽，并与视杆内段质膜的特化区域融合。该区域围绕着连接内段和视杆外段的纤毛，成熟的视紫红质被输送到视杆外段。调节视紫红质载体囊泡出芽、靶向和融合的细胞内分选机制已通过多种方法进行了研究，包括一种新开发的无细胞测定法，该方法可重建囊泡出芽。这些研究揭示了小GTP结合蛋白rab6以及视紫红质的羧基末端结构域在高尔基体后囊泡形成中的重要作用。在本报告中，讨论了它们在视紫红质的高尔基体后运输以及光感受器细胞极性和健康维持中的作用。
