Fukumoto T, Torigoe N, Kawabata S, Murakami M, Uede T, Nishi T, Ito Y, Sugimura K
Department of Bioengineering, Faculty of Engineering, Kagoshima University, Japan.
Nat Biotechnol. 1998 Mar;16(3):267-70. doi: 10.1038/nbt0398-267.
Phage library clones selected by a conformational epitope-recognizing and inhibitory monoclonal antibody may display moieties that mimic a receptor/ligand-like three-dimensional structure. This pseudoreceptor/ligand should be able to bind to natural ligand/receptor molecules. We tested this idea using anti-T cell costimulatory molecule antibodies and successfully isolated phage clones with costimulatory effects on T-cell proliferation. This strategy facilitates the designing of regulatory peptide molecules in the absence of precise information about the structure-function relationships in receptor/ligand interactions.
由构象表位识别性抑制性单克隆抗体筛选出的噬菌体文库克隆可能会展示出模拟受体/配体样三维结构的部分。这种假受体/配体应该能够与天然配体/受体分子结合。我们使用抗T细胞共刺激分子抗体验证了这一想法，并成功分离出对T细胞增殖具有共刺激作用的噬菌体克隆。在缺乏关于受体/配体相互作用中结构-功能关系的确切信息的情况下，该策略有助于设计调节性肽分子。
