Department of Large Animal Medicine, College of Veterinary Medicine, University of Georgia, Athens, Georgia, USA.
Equine Vet J. 2012 Jul;44(4):449-52. doi: 10.1111/j.2042-3306.2011.00453.x. Epub 2011 Sep 1.
Accurate measurement of plasma fibrinogen concentrations is an important tool for assessment of horses with inflammatory diseases.
To determine the precision and accuracy of a benchtop instrument using both fresh and frozen equine plasma by comparing the plasma fibrinogen concentration measured by a benchtop instrument to 2 separate laboratory standard methods (ACL 100 and STA Compact) for fibrinogen measurement.
Accuracy and precision of the VSPro was evaluated using both human fibrinogen standards and samples from horses. Fifty frozen samples from horses with gastrointestinal disease had the fibrinogen concentration measured using the ACL 1000 and the VSPro. Fifty fresh samples were collected from hospitalised horses and fibrinogen concentration was measured using the STA Compact coagulation machine and the VSPro. Correlations for measurements were performed, as well as Bland-Altman analysis.
Coefficients of variability for the VSPro ranged from 7% to 15%. The VSPro fibrinogen values were well correlated to both the ACL 1000 (r = 0.94, P<0.001) and the STA Compact measurements (r = 0.926, P<0.001). Bland-Altman analysis showed a mean bias of -0.83 g/l (95% confidence interval -2.03-0.324 g/l) for the ACL 1000 and a mean bias of -0.024 g/l (95% confidence interval -1.434-1.386 g/l) for the STA Compact.
The VSPro appears to have adequate accuracy and precision for clinical measurement of plasma fibrinogen concentrations.
The VSPro provides a measurement of equine plasma fibrinogen concentration using a benchtop instrument with a rapid test time that has comparable accuracy to the fibrinogen concentration obtained from reference laboratories.
准确测量血浆纤维蛋白原浓度是评估炎症性疾病马的重要工具。
通过比较台式仪器测量的血浆纤维蛋白原浓度与 2 种独立的实验室标准方法（ACL 100 和 STA Compact）测量纤维蛋白原的浓度，确定使用新鲜和冷冻马血浆的台式仪器的精密度和准确性。
使用人纤维蛋白原标准品和马的样本评估 VSPro 的准确性和精密度。使用 ACL 1000 和 VSPro 测量患有胃肠道疾病的 50 份冷冻马样本的纤维蛋白原浓度。从住院马采集 50 份新鲜样本，使用 STA Compact 凝血机制仪和 VSPro 测量纤维蛋白原浓度。对测量值进行相关性分析和 Bland-Altman 分析。
VSPro 的变异系数为 7%至 15%。VSPro 纤维蛋白原值与 ACL 1000（r = 0.94，P<0.001）和 STA Compact 测量值（r = 0.926，P<0.001）均具有良好的相关性。Bland-Altman 分析显示 ACL 1000 的平均偏差为-0.83g/l（95%置信区间-2.03-0.324g/l），STA Compact 的平均偏差为-0.024g/l（95%置信区间-1.434-1.386g/l）。
VSPro 似乎具有足够的准确性和精密度，可用于临床测量血浆纤维蛋白原浓度。
VSPro 使用台式仪器快速测试时间提供马血浆纤维蛋白原浓度的测量值，与参考实验室获得的纤维蛋白原浓度具有可比性。
