大鼠舌下腺和颌下腺的糖鞘脂

The glycosphingolipids of rat sublingual and submaxillary glands.

Slomiany A, Annese C, Slomiany B L

Biochim Biophys Acta. 1976 Aug 23;441(2):316-26. doi: 10.1016/0005-2760(76)90175-2.

Abstract

Glycosphingolipids have been isolated from rat sublingual and submaxillary glands by the procedure involving lipid extraction, column fractionation and thin-layer chromatography. 2. The major neutral glycosphingolipids in rat sublingual and submaxillary glands were monohexosylceramide, dihexosylceramide, tetrahexosylceramide and pentahexosylceramide. Both types of glands exhibited a low content of trihexosylceramide. The fucose-containing glycosphingolipids were not found. 3. The acidic glycosphingolipids in rat sublingual and submaxillary glands were composed of monohexose sulfatide, dihexose sulfatide and monosialo-and disialogangliosides of hematoside series. In addition, small quantities of gangliosides containing hexosamines were also present. 4. The distribution of acidic and neutral glycosphingolipids was similar in the sublingual and submaxillary glands, except for the tetrahexosylceramide and sultatides. Sublingual glands contained 1.5 and 3.0 times as much tetrahexosylceramide and sulfatides, respectively, as did submaxillary glands. 5. The glycosphingolipids of submaxillary and sublingual glands showed large similarity in fatty acid composition. The fatty acid composition of gangliosides resembled each other, but differed remarkably from those of sulfatides and neutral glycosphingolipids in the docosanoate content.

摘要

通过脂质提取、柱分级分离和薄层色谱法，已从大鼠舌下腺和颌下腺中分离出糖鞘脂。2. 大鼠舌下腺和颌下腺中的主要中性糖鞘脂为单己糖神经酰胺、二己糖神经酰胺、四己糖神经酰胺和五己糖神经酰胺。两种腺体中三己糖神经酰胺的含量均较低。未发现含岩藻糖的糖鞘脂。3. 大鼠舌下腺和颌下腺中的酸性糖鞘脂由单己糖硫酸脑苷脂、二己糖硫酸脑苷脂以及血苷系列的单唾液酸和双唾液酸神经节苷脂组成。此外，还存在少量含己糖胺的神经节苷脂。4. 除四己糖神经酰胺和硫酸脑苷脂外，舌下腺和颌下腺中酸性和中性糖鞘脂的分布相似。舌下腺中四己糖神经酰胺和硫酸脑苷脂的含量分别是颌下腺的1.5倍和3.0倍。5. 颌下腺和舌下腺的糖鞘脂在脂肪酸组成上有很大相似性。神经节苷脂的脂肪酸组成彼此相似，但在二十二碳烷酸含量上与硫酸脑苷脂和中性糖鞘脂有显著差异。