Waring A J, Breidenbach R W, Lyons J M
Biochim Biophys Acta. 1976 Aug 16;443(2):157-68. doi: 10.1016/0005-2736(76)90499-5.
Tomato seedlings treated with ethanolamine showed altered phospholipid composition. The changes included altered acyl chain composition as well as changes in the relative amounts of the phospholipid classes. Specifically, there was an increase in phosphatidylethanolamine and phosphatidylserine with a concomitant decrease in phosphatidylcholine and no overall increase in phospholipids. Treatment with ethanolamine increased the relative amount of C18 acyl chains (especially 18 : 2) in phosphatidylethanolamine and phosphatidylcholine at the expense of 16 : 0 and 16 : 1. Acyl composition of other phospholipid classes were unchanged. Labeled ethanolamine was incorporated mostly into phosphatidylethanolamine and phosphatidylcholine. Ethanolamine-stimulated incorporation of labeled oleate was entirely into acyl chains and appeared only as 18 : 1 and 18 : 2. There was greater incorporation, but less conversion of 18 : 1 to 18 : 2 with choline. Stearate was incorporated but desaturated.
用乙醇胺处理的番茄幼苗显示出磷脂组成的改变。这些变化包括酰基链组成的改变以及磷脂类相对含量的变化。具体而言,磷脂酰乙醇胺和磷脂酰丝氨酸增加,同时磷脂酰胆碱减少,且磷脂总量没有总体增加。用乙醇胺处理增加了磷脂酰乙醇胺和磷脂酰胆碱中C18酰基链(尤其是18:2)的相对含量,代价是16:0和16:1减少。其他磷脂类的酰基组成未发生变化。标记的乙醇胺主要掺入磷脂酰乙醇胺和磷脂酰胆碱中。乙醇胺刺激的标记油酸酯掺入完全进入酰基链,且仅以18:1和18:2出现。胆碱的掺入量更大,但18:1向18:2的转化率较低。硬脂酸被掺入但发生了去饱和作用。