Department of Plant Physiology, University of Groningen, Biological Centre, P. O. Box 14, NL-9750 AA, Haren(Gn), The Netherlands.
Planta. 1985 May;164(1):83-8. doi: 10.1007/BF00391029.
The effect of pretreatment of cucumber (Cucumis sativus L.) roots with choline chloride or ethanolamine on leaf phospholipid composition and light-induced leaf damage during chilling was studied. Photooxidative chlorophyll degradation was similarly inhibited by both amino alcohols. The decrease of the chlorophyll a/chlorophyll b ratio and the increase of polyunsaturated-fatty-acid degradation during chilling in the light were equally inhibited by pretreatment with choline chloride or ethanolamine. Treatment with choline chloride and ethanolamine caused, respectively, 43% and 26% increases in the total phospholipid contents of the leaves. After treatment with choline chloride, the phosphatidylcholine content was higher than the content of phosphatidylethanolamine; the reverse was true after treatment with ethanolamine. The chlorophyll concentration increased less than the phospholipid concentration, resulting in a decreased chlorophyll/phospholipid ratio of treated leaves. During chilling in the light, degradation of phosphatidylcholine, ethanolamine and phosphatidyl glycerol occurred. Phosphatidyl glycerol was less sensitive than phosphatidylcholine and ethanolamine. The degradation was equally inhibited by pretreatment with either amino alcohol. Possible connections between the phospholipid content of leaf membranes and the inhibition of chilling-induced photooxidative leaf damage are discussed.
用氯化胆碱或乙醇胺预处理黄瓜（ Cucumis sativus L. ）根对低温下叶片磷脂组成和光诱导叶片损伤的影响。两种氨基醇都能相似地抑制光氧化叶绿素降解。在光照下低温处理时，叶绿素 a/叶绿素 b 比值降低，多不饱和脂肪酸降解增加，这两种情况都受到氯化胆碱或乙醇胺预处理的同样抑制。用氯化胆碱和乙醇胺处理，叶片的总磷脂含量分别增加了 43%和 26%。用氯化胆碱处理后，磷脂酰胆碱的含量高于磷脂酰乙醇胺的含量；用乙醇胺处理后则相反。叶绿素浓度的增加低于磷脂浓度，导致处理叶片的叶绿素/磷脂比降低。在光照下低温处理时，发生了磷脂酰胆碱、乙醇胺和磷脂酰甘油的降解。磷脂酰甘油比磷脂酰胆碱和乙醇胺更不敏感。用氨基醇预处理均可同样抑制这种降解。讨论了叶片膜磷脂含量与抑制低温诱导光氧化叶片损伤之间的可能联系。
