Rangel S R, Grief C, Da Silva E E, de Filippis A M, Taffarel M
Department of Virology, IOC/FIOCRUZ, Rio de Janeiro, Brazil.
J Submicrosc Cytol Pathol. 1998 Jan;30(1):71-5.
Rhabdomyosarcoma monolayers were inoculated with enterovirus 71 (EV 71) at 73 degrees C, sampled at intervals during the replicative cycle, and examined in thin sections by electron microscopy, using routine and immunoelectronmicroscopy with polyclonal antibodies against EV 71. The location of EV 71 or its precursors was followed during the viral replicative cycle. The earliest samples (3 h postinoculation) showed a cell shape change, from elongated to rounded. At 6 h postinoculation, the presence of early virus-induced vesicles developing within the cytoplasm was pointed out, although no virus particles were observed at these stages. At 12 and 20 h postinoculation, virus particles appeared in the cytoplasm. They were found free or in clusters in the cytoplasmic matrix, between the virus-induced vesicles. EV 71 particles were also occasionally observed in the form of paracrystalline arrays situated in the vesiculated areas. The immunolabel (gold beads) was found, initially, over dense cytoplasmic areas and in more advanced process at the vesiculated area and over the virus particles. Therefore the main cellular alterations observed in this infection were the shape change of the cell and the appearance of electron-dense areas (viroplasm) and smooth walled vesicles which are probably the site of the virus replication.
横纹肌肉瘤单层细胞在73摄氏度下接种肠道病毒71型（EV 71），在复制周期中定期取样，并通过电子显微镜在薄切片中进行检查，使用针对EV 71的多克隆抗体进行常规和免疫电子显微镜检查。在病毒复制周期中追踪EV 71或其前体的位置。最早的样本（接种后3小时）显示细胞形状发生变化，从细长形变为圆形。接种后6小时，指出在细胞质内出现早期病毒诱导的囊泡，尽管在这些阶段未观察到病毒颗粒。接种后12小时和20小时，病毒颗粒出现在细胞质中。它们在细胞质基质中游离或成簇存在，位于病毒诱导的囊泡之间。EV 71颗粒偶尔也以位于囊泡化区域的准晶体阵列形式观察到。免疫标记（金珠）最初在致密的细胞质区域发现，在更晚期的过程中出现在囊泡化区域和病毒颗粒上。因此，在这种感染中观察到的主要细胞改变是细胞形状变化以及电子致密区域（病毒质）和平滑壁囊泡的出现，这些可能是病毒复制的部位。
