用于酵母抗真菌药敏试验的E试验与肉汤微量稀释法的比较。

Comparison of the E test with broth microdilution for antifungal susceptibility testing of yeasts.

作者信息

Gould I M, Milne K, Wood W, Kunova A

机构信息

Dept. of Microbiology, Royal Infirmary, Foresterhill, Aberdeen, UK.

出版信息

J Chemother. 1998 Feb;10(1):3-6. doi: 10.1179/joc.1998.10.1.3.

DOI:10.1179/joc.1998.10.1.3

PMID:9531068

Abstract

The susceptibilities of 26 recent invasive clinical isolates to amphotericin B (AMP), 5-flucytosine (5FC), fluconazole (FLU) and itraconazole (ITR) were determined by a broth microdilution modification of the NCCLS M27P method and also by E test. Using breakpoint criteria each result was classified as either sensitive (S), intermediate (I) or resistant (R). Taking the optical density (OD)80 as the standard, the results were further classified into major (M) or minor (m) errors. E test: AMP = 0M 0m, 5FC = 0M 5m, FLU = 1M 12m, ITR = 1M 5m errors. Minimal inhibitory concentrations (MIC): AMP = 0M 2m, 5FC = 0M 0m, FLU = 3M 4m, ITR = 1M 7m errors. The E test was quick and relatively simple to perform. Results using the E test compared favourably with those of the OD80 and it should be suitable for the routine susceptibility testing of yeasts to antifungal agents.

摘要

采用美国国家临床实验室标准化委员会（NCCLS）M27 - P方法的肉汤微量稀释改良法并结合E试验，测定了26株近期侵袭性临床分离株对两性霉素B（AMP）、5 - 氟胞嘧啶（5FC）、氟康唑（FLU）和伊曲康唑（ITR）的敏感性。根据折点标准，将每个结果分类为敏感（S）、中介（I）或耐药（R）。以光密度（OD）80为标准，结果进一步分为主要（M）或次要（m）误差。E试验：AMP = 0个主要误差0个次要误差，5FC = 0个主要误差5个次要误差，FLU = 1个主要误差12个次要误差，ITR = 1个主要误差5个次要误差。最小抑菌浓度（MIC）：AMP = 0个主要误差2个次要误差，5FC = 0个主要误差0个次要误差，FLU = 3个主要误差4个次要误差，ITR = 1个主要误差7个次要误差。E试验操作快速且相对简单。E试验的结果与OD80法的结果相比具有优势，它应适用于酵母菌对抗真菌药物的常规药敏试验。