人钠/碘同向转运体基因功能性启动子的克隆
Cloning of a functional promoter of the human sodium/iodide-symporter gene.
作者信息
Behr M, Schmitt T L, Espinoza C R, Loos U
机构信息
Abteilung Innere Medizin I, University of Ulm, Universitätsklinikum Ulm, Robert-Koch-Strasse 8, 89081 Ulm, Federal Republic of Germany.
出版信息
Biochem J. 1998 Apr 15;331 ( Pt 2)(Pt 2):359-63. doi: 10.1042/bj3310359.
Abstract
We have cloned and sequenced genomic DNA from a human library extending 1300 bp upstream the 5'-untranslated sequence of the cDNA coding for the sodium/iodide symporter. In transient transfection assays this sequence exhibited promoter activity, which could be confined to nucleotides -443 to -395 relative to the ATG start codon. This minimal promoter, including a putative GC- and TATA- box, was preferentially activated in the rat thyroid cell line FRTL-5, but was also active in non-thyroidal cells, such as COS-7 and Chinese-hamster ovary, albeit to a markedly lower extent.
摘要
我们从一个人类文库中克隆并测序了基因组DNA，该文库延伸至编码钠/碘同向转运体的cDNA的5'-非翻译序列上游1300 bp处。在瞬时转染实验中，该序列表现出启动子活性，其活性可局限于相对于ATG起始密码子的-443至-395核苷酸处。这个最小启动子，包括一个假定的GC盒和TATA盒，在大鼠甲状腺细胞系FRTL-5中被优先激活，但在非甲状腺细胞如COS-7和中国仓鼠卵巢细胞中也有活性，尽管活性程度明显较低。
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本文引用的文献
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