Chen S U, Chao K H, Wu M Y, Chen C D, Ho H N, Yang Y S
Department of Obstetrics and Gynecology, College of Medicine, Taipei, Taiwan.
Fertil Steril. 1998 Mar;69(3):569-75. doi: 10.1016/s0015-0282(97)00535-9.
To evaluate the efficiency and efficacy of a simplified two-pipette technique in comparison to the conventional three-pipette method; in the two-pipette method, a single, larger drilling/biopsy pipette is used to perform zona pellucida (ZP) drilling and blastomere aspiration for embryo biopsy.
A preclinical, prospective, randomized, in vitro experiment.
The reproductive unit of a university teaching hospital.
PATIENT(S): Ninety-five excess embryos at the two- to four-cell stage were obtained from 35 patients undergoing IVF.
INTERVENTION(S): At the six- to eight-cell stage, 88 embryos were allocated randomly to three groups: group I for the conventional method (n = 29), group II for the simplified technique (n = 30), and group III for controls (n = 29). The embryos then were cultured in vitro. The retrieved blastomeres were fixed and examined with fluorescence in situ hybridization using X and Y probes simultaneously.
MAIN OUTCOME MEASURE(S): Biopsy time, successful retrieval of a blastomere, fixation of the cell, signals developed from fluorescence in situ hybridization, and growth potential and hatching capacity of the biopsied embryos were evaluated.
RESULT(S): The mean time (+/- SD) for biopsy of each embryo in group I (435 +/- 137 seconds) was significantly longer than that in group II (126 +/- 32 seconds). The success rates for obtaining an intact blastomere were not different between group I (93%) and group II (97%). The growth capacity to the blastocyst stage was similar among the three groups (34%, 37%, and 38%, respectively). However, the ZP-drilled and biopsied embryos of groups I and II had higher percentages of hatching (34% and 37%, respectively) and complete hatching (17% and 20%, respectively) than did those of group III (10% and 0, respectively). The blastomeres obtained by biopsy in groups I and II were equally fixed (90% vs. 90%, respectively) and shown in fluorescence in situ hybridization (79% vs. 80%, respectively).
CONCLUSION(S): Compared with the conventional method, the simplified technique is more efficient and equally efficacious for blastomere biopsy in preimplantation genetic diagnosis.
与传统的三吸管法相比，评估简化双吸管技术的效率和效果；在双吸管法中，使用单个较大的钻孔/活检吸管进行透明带（ZP）钻孔和卵裂球抽吸以进行胚胎活检。
一项临床前、前瞻性、随机、体外实验。
一所大学教学医院的生殖科。
从35例行体外受精（IVF）的患者中获得95个处于二至四细胞阶段的多余胚胎。
在六至八细胞阶段，将88个胚胎随机分为三组：第一组采用传统方法（n = 29），第二组采用简化技术（n = 30），第三组为对照组（n = 29）。然后将胚胎进行体外培养。回收的卵裂球进行固定，并同时使用X和Y探针通过荧光原位杂交进行检查。
评估活检时间、成功获取卵裂球、细胞固定情况、荧光原位杂交产生的信号以及活检后胚胎的生长潜力和孵化能力。
第一组每个胚胎活检的平均时间（±标准差）（435 ± 137秒）显著长于第二组（126 ± 32秒）。第一组（93%）和第二组（97%）获取完整卵裂球的成功率无差异。三组发育至囊胚阶段的生长能力相似（分别为34%、37%和38%）。然而，第一组和第二组经ZP钻孔和活检的胚胎孵化率（分别为34%和37%）和完全孵化率（分别为17%和20%）高于第三组（分别为10%和0）。第一组和第二组活检获得的卵裂球固定情况相同（分别为90%对90%），荧光原位杂交显示情况也相同（分别为79%对80%）。
与传统方法相比，简化技术在植入前基因诊断中对卵裂球活检更高效且效果相同。
