Currie L M, Livesey S A, Harper J R, Connor J
LifeCell Corporation, The Woodlands, Texas, USA.
Transfusion. 1998 Feb;38(2):160-7. doi: 10.1046/j.1537-2995.1998.38298193098.x.
The potential for bacterial contamination limits the storage of platelets at 22 degrees C to 5 days. This creates an inventory problem, which could be overcome by the use of cryopreservation to allow long-term storage of platelets. It has been demonstrated that the addition to platelets of a mixture of second-messenger effectors (platelet storage solution), allows these cells to retain significant in vitro functional activity following cold storage. Analysis is needed of the ability of this second messenger effector mixture both to protect platelets during cryopreservation and to reduce the need for a cryoprotectant.
Fresh single-donor platelet units (n = 8) were divided into three samples and treated with 6-percent dimethyl sulfoxide (DMSO), 2-percent DMSO or the platelet storage solution and 2-percent DMSO. The samples were placed directly into a -80 degrees C freezer and stored for 1 week, after which they were thawed and analyzed for in vitro functional activity.
Platelets cryopreserved with the platelet storage solution and 2-percent DMSO displayed statistically higher retention of functional activity and viability--including cell number, percent of discoid cells, extent of shape change, and hypotonic shock response--than did platelets stored by the method using 6-percent DMSO. In addition, the treated platelets displayed statistically lower expression of p-selectin. The treated platelets showed no loss of cell number, > 88-percent retention of discoid morphology, and > 75-percent retention of ristocetin-induced aggregation as compared to values for these measures in fresh platelets.
The use of this platelet storage solution in the cryopreservation of platelets yields a significant improvement in their postthaw in vitro recovery and allows for a reduction of the DMSO concentration from 6 to 2 percent, with superior maintenance of in vitro viability and function.
细菌污染的可能性限制了血小板在22℃下的储存时间为5天。这就产生了库存问题，而通过使用冷冻保存来实现血小板的长期储存或许可以解决这一问题。已经证明，向血小板中添加第二信使效应物混合物（血小板保存溶液），可使这些细胞在冷藏后仍保留显著的体外功能活性。需要分析这种第二信使效应物混合物在血小板冷冻保存过程中保护血小板以及减少对冷冻保护剂需求的能力。
将新鲜的单供体血小板单位（n = 8）分成三个样本，分别用6%二甲基亚砜（DMSO）、2% DMSO或血小板保存溶液加2% DMSO处理。样本直接放入-80℃冰箱中储存1周，之后解冻并分析其体外功能活性。
用血小板保存溶液加2% DMSO冷冻保存的血小板在功能活性和活力方面的保留率在统计学上高于使用6% DMSO方法保存的血小板，包括细胞数量、盘状细胞百分比、形状变化程度和低渗休克反应。此外，处理后的血小板在统计学上p-选择素的表达更低。与新鲜血小板相比，处理后的血小板细胞数量没有损失，盘状形态保留率> 88%，瑞斯托霉素诱导的聚集保留率> 75%。
在血小板冷冻保存中使用这种血小板保存溶液可显著改善其解冻后的体外恢复情况，并可将DMSO浓度从6%降至2%，同时能更好地维持体外活力和功能。
