Tanihara M, Suzuki Y, Nishimura Y, Suzuki K, Kakimaru Y
Graduate School of Material Science, NARA Institute of Science and Technology, Ikoma, Japan.
Peptides. 1998;19(3):421-5. doi: 10.1016/s0196-9781(97)00420-8.
We have previously reported an elevation of thrombin-like activity in infected wound exudates. Therefore, using this enzymatic activity as a biological signal, a system which can release an antimicrobial drug at infected wounds was investigated. In this paper, we report thrombin-sensitive peptide linkers, the key component of this system. Starting from amino acid sequences of the cleavage site in fibrinogen, which is the substrate of thrombin, we synthesized some thrombin-sensitive peptide linkers. We constructed devices in which the thrombin-sensitive peptide linker interconnected between polyvinyl alcohol hydrogel and gentamicin. The device was able to release gentamicin in response to thrombin.
我们之前报道过感染伤口渗出液中类凝血酶活性升高。因此，利用这种酶活性作为生物信号，研究了一种可在感染伤口处释放抗菌药物的系统。在本文中，我们报道了凝血酶敏感肽接头，这是该系统的关键组成部分。从凝血酶底物纤维蛋白原裂解位点的氨基酸序列出发，我们合成了一些凝血酶敏感肽接头。我们构建了凝血酶敏感肽接头连接聚乙烯醇水凝胶和庆大霉素的装置。该装置能够响应凝血酶释放庆大霉素。
