Equipe de Recherche sur les Relations Matrice Extracellulaire Cellules (Errmece), ‡Laboratoire de Physicochimie des Polymères et des Interfaces (LPPI), Institut des Matériaux, University of Cergy-Pontoise , 95000 Cergy-Pontoise, France.
Biomacromolecules. 2013 Nov 11;14(11):3870-9. doi: 10.1021/bm400991k. Epub 2013 Oct 8.
A fibrin hydrogel at physiological concentration (5 mg/mL) was associated with polyvinyl alcohol (PVA) inside an interpenetrating polymer networks (IPN) architecture. Previously, PVA has been modified with methacrylate functions in order to cross-link it by free-radical polymerization. The fibrin network was synthesized by the enzymatic hydrolysis of fibrinogen by thrombin. The resulting self-supported materials simultaneously exhibit the properties of the fibrin hydrogel and those of the synthetic polymer network. Their storage modulus is 50-fold higher than that of the fibrin hydrogel and they are completely rehydratable. These materials are noncytotoxic toward human fibroblast and the fibrin present on the surface of PVAm-based IPNs favors cell development.
一种生理浓度（5mg/mL）的纤维蛋白水凝胶与聚乙烯醇（PVA）在互穿聚合物网络（IPN）结构中结合。先前，PVA 已通过甲基丙烯酰基功能化来进行改性，以便通过自由基聚合进行交联。纤维蛋白网络通过凝血酶对纤维蛋白原的酶解来合成。所得的自支撑材料同时具有纤维蛋白水凝胶和合成聚合物网络的性质。其储能模量比纤维蛋白水凝胶高 50 倍，且完全可再水合。这些材料对人成纤维细胞无细胞毒性，并且存在于基于 PVAm 的 IPN 表面的纤维蛋白有利于细胞发育。
