Coffey T J, Enright M C, Daniels M, Wilkinson P, Berrón S, Fenoll A, Spratt B G
Molecular Microbiology Group, School of Biological Sciences, University of Sussex, Brighton, UK.
Microb Drug Resist. 1998 Spring;4(1):51-5. doi: 10.1089/mdr.1998.4.51.
Multiply-antibiotic-resistant isolates of serogroup 19 Streptococcus pneumoniae, possessing altered penicillin-binding protein (PBP) 1A, 2B, and 2X genes that are indistinguishable from those of the Spanish multiresistant serogroup 23F clone, are now commonly encountered in Spain. Those isolates that have been serotyped express type 19F capsular polysaccharide. Serotyping of further isolates, and hybridization using a serotype 19F-specific probe, has shown that some of them are serotype 19A, rather than 19F. The Spanish multiresistant serotype 19A, 19F, and 23F multiresistant strains were all shown to be very closely related in overall genotype, as they were indistinguishable by REP-PCR and by the sequencing of internal fragments of three house-keeping genes. The serotype 19A multiresistant strains, like the serotype 19F multiresistant strains, therefore appear to be a serotype variant of the Spanish multiresistant serotype 23F clone, which presumably has arisen by recombination at the capsular locus.
在西班牙，现在经常遇到19血清群肺炎链球菌的多重耐药菌株，这些菌株具有与西班牙多重耐药23F血清群克隆株无法区分的青霉素结合蛋白（PBP）1A、2B和2X基因改变。那些已进行血清分型的分离株表达19F型荚膜多糖。对更多分离株进行血清分型，并使用19F血清型特异性探针进行杂交，结果表明其中一些是19A血清型，而非19F血清型。西班牙多重耐药19A、19F和23F多重耐药菌株在总体基因型上都显示出非常密切的关系，因为通过重复序列PCR（REP-PCR）和三个管家基因内部片段测序无法区分它们。因此，19A血清型多重耐药菌株与19F血清型多重耐药菌株一样，似乎是西班牙多重耐药23F血清群克隆株的血清型变体，推测是通过荚膜位点的重组产生的。
