Meddows-Taylor S, Martin D J, Tiemessen C T
Medical Research Council AIDS Virus Research Unit, National Institute for Virology, Johannesburg, South Africa.
J Infect Dis. 1998 Apr;177(4):921-30. doi: 10.1086/515232.
The expression of the two human interleukin (IL)-8 receptors, designated IL-8RA (CXCR-1) and IL-8RB (CXCR-2), on the surface of whole blood polymorphonuclear leukocytes (PMNL) was determined by use of receptor-specific monoclonal antibodies and flow cytometry. Sixteen subjects each were included in 4 study groups: healthy blood donors (ND), patients with pulmonary tuberculosis (TB), human immunodeficiency virus type 1-seropositive patients (HIV), and HIV-1-seropositive subjects with pulmonary tuberculosis (HIV/TB). A significant reduction in the percentage of PMNL expressing IL-8RA and IL-8RB and in their respective fluorescence intensities was found in TB, HIV, and HIV/TB groups compared with that obtained for the ND group. The greatest down-regulation of both receptors occurred in the HIV/TB group. Furthermore, associated with this reduced expression of IL-8 receptors was impairment of both intracellular calcium flux and migration of PMNL in response to IL-8 in a group of HIV/TB patients compared with that in healthy persons.
通过使用受体特异性单克隆抗体和流式细胞术，测定了全血多形核白细胞（PMNL）表面两种人类白细胞介素（IL）-8受体的表达，这两种受体分别命名为IL-8RA（CXCR-1）和IL-8RB（CXCR-2）。4个研究组每组纳入16名受试者：健康献血者（ND）、肺结核患者（TB）、1型人类免疫缺陷病毒血清阳性患者（HIV）以及合并肺结核的HIV-1血清阳性受试者（HIV/TB）。与ND组相比，TB组、HIV组和HIV/TB组中表达IL-8RA和IL-8RB的PMNL百分比及其各自的荧光强度均显著降低。两种受体下调最明显的是HIV/TB组。此外，与IL-8受体表达降低相关的是，一组HIV/TB患者的PMNL细胞内钙流和对IL-8的迁移反应均较健康人受损。
