Goldblatt D, Vaz A R, Miller E
Institute of Child Health, and Immunisation Division, Communicable Disease Surveillance Centre, London, United Kingdom.
J Infect Dis. 1998 Apr;177(4):1112-5. doi: 10.1086/517407.
Evaluation of the new generation of conjugate vaccines is hampered by the absence of reliable surrogate markers of immunologic memory. Memory responses are characterized by rapid production of relatively high-avidity antibody; thus, a solid-phase ELISA was adapted for the measurement of anti-Haemophilus influenzae type b (Hib) IgG avidity. In a cohort of infants vaccinated at 2, 3, and 4 months of age with Hib conjugate vaccines, avidity increased in the period following vaccination, while antibody titer fell. After a booster dose at 1 year of age, both antibody titer and avidity increased. In a cohort with anti-Hib IgG <1.0 microg/mL following primary immunization, antibody avidity after booster was low, indicating an absence of priming. Antibody avidity may help distinguish, in persons with low antibody titers, between those who are primed for memory and those who are not.
新一代结合疫苗的评估因缺乏可靠的免疫记忆替代标志物而受阻。记忆反应的特征是快速产生相对高亲和力的抗体；因此，一种固相酶联免疫吸附测定法（ELISA）被用于测量抗b型流感嗜血杆菌（Hib）IgG的亲和力。在一组2、3、4月龄接种Hib结合疫苗的婴儿中，接种后亲和力增加，而抗体滴度下降。在1岁时进行加强免疫后，抗体滴度和亲和力均增加。在初次免疫后抗Hib IgG<1.0μg/mL的一组人群中，加强免疫后的抗体亲和力较低，表明未发生致敏。抗体亲和力可能有助于在抗体滴度较低的人群中区分已产生记忆的人群和未产生记忆的人群。
