Kamboj K K, King C L, Greenspan N S, Kirchner H L, Schreiber J R
Department of Pediatrics, Case Western Reserve University, Cleveland, Ohio, USA.
J Infect Dis. 2001 Oct 1;184(7):931-5. doi: 10.1086/323342. Epub 2001 Aug 22.
Haemophilus influenzae type b (Hib) capsular polysaccharide (PS) induces protective antibodies but is T independent and poorly immunogenic in infants. Conjugate vaccines of Hib PS linked to proteins, such as CRM(197), increase the PS antibody titer and elicit immunologic memory. To define the conjugate-induced memory T cell response, 19 adults were immunized with Hib-CRM(197), and antibody titers, carrier protein-specific CD4(+) T cell proliferation, and cytokine production were measured. Hib-CRM(197) induced PS and CRM(197) antibodies, vigorous T cell recall responses, and production of cytokines, including interleukin (IL)-2, IL-5, IL-10, and interferon-gamma. There was marked variability in PS antibody titer, despite consistent CRM(197)-specific recall responsiveness, which correlated with peak IgM and IgA PS antibody titers. Correlations were also found between IL-2 and IL-5 and IgA PS antibody levels. Hib-CRM(197) induced a rapid increase in CRM(197)-specific memory T cells and mixed Th1/Th2 cytokines, which may regulate the isotype and quantity of PS antibody.
b型流感嗜血杆菌（Hib）的荚膜多糖（PS）可诱导产生保护性抗体，但它是T细胞非依赖性的，在婴儿中免疫原性较差。与蛋白质（如CRM197）连接的Hib PS结合疫苗可提高PS抗体滴度并引发免疫记忆。为了确定结合疫苗诱导的记忆T细胞反应，对19名成年人接种了Hib-CRM197，并检测了抗体滴度、载体蛋白特异性CD4(+) T细胞增殖和细胞因子产生情况。Hib-CRM197诱导产生了PS和CRM197抗体、强烈的T细胞回忆反应以及包括白细胞介素（IL）-2、IL-5、IL-10和干扰素-γ在内的细胞因子的产生。尽管CRM197特异性回忆反应一致，但PS抗体滴度存在显著差异，这与IgM和IgA PS抗体滴度峰值相关。还发现IL-2和IL-5与IgA PS抗体水平之间存在相关性。Hib-CRM197诱导CRM197特异性记忆T细胞快速增加以及Th1/Th2细胞因子混合产生，这可能调节PS抗体的同种型和数量。
