Misra S, Mukherjee M, Dikshit M, Chatterjee R K
Division of Parasitology, Central Drug Research Institute, Lucknow, India.
Trop Med Int Health. 1998 Feb;3(2):124-9. doi: 10.1046/j.1365-3156.1998.00161.x.
To determine mitogenic and antigen-specific cellular immune responses of two species of rodents, viz. Meriones unguiculatus and Mastomys coucha to assess the usefulness of the A. viteae/Mastomys model for cellular immune studies in experimental filariasis.
Lymphocyte blast transformation test (LTT) using spleen cells of normal and A. viteae infected animals.
The proliferative response of gerbils was much higher than that of Mastomys to both ConA and filarial antigens. Cells of both species of rodents did not respond to microfilarial (mf) antigen, however, their mitogenic response differed during infection. Some degree of nonspecific suppression was observed in gerbils during prepatent and patent stages of infection, while Mastomys revealed highest proliferation during patent microfilaraemia. Mastomys cells did not respond to adult or mf antigen, while adult-specific proliferation was detected in the case of gerbils.
The A. viteae/gerbil model shows more similarity to human filarial infection regarding cellular immune response. Markedly low responsiveness of a high percentage of Mastomys and wide variations in the cellular response to nonspecific mitogen limit the usefulness of Mastomys coucha in immunological studies, especially cellular immunity.
确定两种啮齿动物，即长爪沙鼠和南非多乳鼠的促有丝分裂和抗原特异性细胞免疫反应，以评估罗阿丝虫/南非多乳鼠模型在实验性丝虫病细胞免疫研究中的实用性。
使用正常和感染罗阿丝虫动物的脾细胞进行淋巴细胞增殖转化试验（LTT）。
沙鼠对刀豆蛋白A（ConA）和丝虫抗原的增殖反应均远高于南非多乳鼠。两种啮齿动物的细胞对微丝蚴（mf）抗原均无反应，不过，它们在感染期间的促有丝分裂反应有所不同。在感染的潜伏期和成虫期，沙鼠出现了一定程度的非特异性抑制，而南非多乳鼠在微丝蚴血症期的增殖最为明显。南非多乳鼠细胞对成虫或微丝蚴抗原均无反应，而沙鼠细胞则出现了成虫特异性增殖。
罗阿丝虫/沙鼠模型在细胞免疫反应方面与人类丝虫感染表现出更多相似性。南非多乳鼠的高比例低反应性以及对非特异性有丝分裂原的细胞反应的广泛差异限制了南非多乳鼠在免疫研究，尤其是细胞免疫研究中的实用性。
