Shibata K, Hasebe A, Sasaki T, Watanabe T
Department of Oral Bacteriology, Hokkaido University School of Dentistry, Sapporo, Japan.
FEMS Immunol Med Microbiol. 1997 Dec;19(4):275-83. doi: 10.1111/j.1574-695X.1997.tb01097.x.
Analysis by an enzyme-linked immunosorbent assay for cytokines indicated that whole cells, intracellular materials and cell membranes of Mycoplasma salivarium induced interleukin-6 and interleukin-8 in a human gingival fibroblast cell line, Gin-1 cells. This was confirmed by reverse transcription-polymerase chain reaction analysis of mRNAs of these cytokines. Studies with inhibitors of second-messenger pathway indicated that a protein kinase C-dependent pathway was involved in the expression of the activity of the cell membranes. In addition, whole cells of other mycoplasmas (M. hominis, M. arthritidis, M. arginini, M. fermentans, M. penetrans, M. pirum and M. pneumoniae) tested for comparative purposes were also shown to possess the activity. Thus, this study demonstrated that mycoplasmas possess the activity to induce interleukin-6 and interleukin-8 in human fibroblasts.
通过酶联免疫吸附测定法对细胞因子进行分析表明，唾液支原体的全细胞、细胞内物质和细胞膜可在人牙龈成纤维细胞系Gin-1细胞中诱导白细胞介素-6和白细胞介素-8的产生。这通过对这些细胞因子的mRNA进行逆转录-聚合酶链反应分析得到了证实。对第二信使途径抑制剂的研究表明，蛋白激酶C依赖性途径参与了细胞膜活性的表达。此外，为作比较而检测的其他支原体（人型支原体、关节炎支原体、精氨酸支原体、发酵支原体、穿透支原体、梨支原体和肺炎支原体）的全细胞也显示具有该活性。因此，本研究证明支原体具有在人成纤维细胞中诱导白细胞介素-6和白细胞介素-8的活性。
