Scarpi C, Ninci F, Centini M, Anselmi C
U.O. Chimica Ambientale I, ARPAT (Agenzia Regionale per la Protezione Ambientale della Toscana), Dipartimento Provinciale di Firenze, Italy.
J Chromatogr A. 1998 Feb 20;796(2):319-25. doi: 10.1016/s0021-9673(97)01015-7.
A simple and reliable HPLC method is described for the simultaneous determination of nine direct and temporary hair dyes in hair colourings containing vegetal extracts. Detection was performed by a diode array detector and two different wavelengths, in the visible range (450 and 650 nm), were used for quantitation. The method does not involve any extraction procedure and it is sufficiently rapid and accurate for routine analyses. The method described was successfully applied to the identification of synthetic organic dyes in 13 direct and temporary hair dyeing formulations commercialized as 'natural'.
描述了一种简单可靠的高效液相色谱法，用于同时测定含植物提取物的染发剂中的九种直接和暂时染发剂。检测由二极管阵列检测器进行，定量采用可见光范围内的两个不同波长（450和650 nm）。该方法无需任何萃取步骤，对于常规分析而言足够快速且准确。所描述的方法已成功应用于鉴定13种作为“天然”产品商业化的直接和暂时染发剂配方中的合成有机染料。
