通过色谱法鉴定酒精诱导的血红蛋白加合物，作为女性酒精滥用的标志物。

Chromatographically identified alcohol-induced haemoglobin adducts as markers of alcohol abuse among women.

作者信息

Hurme L, Seppä K, Rajaniemi H, Sillanaukee P

机构信息

Pharmacia & Upjohn, Uppsala, Sweden.

出版信息

Eur J Clin Invest. 1998 Feb;28(2):87-94. doi: 10.1046/j.1365-2362.1998.00258.x.

DOI:10.1046/j.1365-2362.1998.00258.x

PMID:9541121

Abstract

BACKGROUND

Alcohol-induced changes in haemoglobin have been suggested as potential biochemical markers of alcohol abuse. In this study, we investigated the diagnostic value of alcohol-induced haemoglobin adducts among women.

METHODS

Whole (Hb fractions) and affinity-purified (AHb fractions) haemolysates from 87 women in three groups (a) social drinkers (n=31), (b) heavy drinkers (n=27) and (c) alcoholic subjects (n=29) - were analysed by HPLC-CEC.

RESULTS

Three fractions (HbA 1a2, HbA1dl and AHbA1d1) showed significant differences (P<0.05) between the groups and a significant positive correlation (P<0.05) with self-reported alcohol consumption (r=0.58-0.76) as determined by the Malmö modified Michigan Alcoholism Screening Test (MmMAST) and structured CAGE questionnaire (r=0.58-0.76). HbA1a2, HbA1d1 and AHbA1d1 had specificities of 97%, 97% and 100% respectively and detected 41%, 33% and 78% of heavy drinkers with overall accuracies (OAs) of 71%, 67% and 90%. Sensitivities in the detection of alcoholic subjects were 86% (OA=92%), 76% (OA=87%) and 81 % (OA=91%) respectively. The fractions had higher OAs than traditional markers of alcohol abuse.

CONCLUSION

This study indicates that at least three alcohol-induced haemoglobin adducts occurring in vivo can be measured with promising diagnostic efficiency among women.

摘要

背景

酒精引起的血红蛋白变化被认为是酒精滥用的潜在生化标志物。在本研究中，我们调查了酒精诱导的血红蛋白加合物在女性中的诊断价值。

方法

通过高效液相色谱 - 毛细管电色谱法分析了三组87名女性的全血（血红蛋白组分）和亲和纯化的（加合血红蛋白组分）溶血产物，这三组分别为：（a）社交饮酒者（n = 31），（b）重度饮酒者（n = 27）和（c）酒精中毒受试者（n = 29）。

结果

三组之间的三个组分（HbA 1a2、HbA1dl和AHbA1d1）显示出显著差异（P < 0.05），并且与自我报告的酒精摄入量呈显著正相关（P < 0.05）（r = 0.58 - 0.76），自我报告的酒精摄入量通过马尔默改良密歇根酒精中毒筛查测试（MmMAST）和结构化CAGE问卷确定（r = 0.58 - 0.76）。HbA1a2、HbA1d1和AHbA1d1的特异性分别为97%、97%和100%，检测出重度饮酒者的比例分别为41%、33%和78%，总体准确率（OA）分别为71%、67%和90%。检测酒精中毒受试者的敏感性分别为86%（OA = 92%）、76%（OA = 87%）和81%（OA = 91%）。这些组分的总体准确率高于传统的酒精滥用标志物。