Kariwa H, Fujiki M, Yoshimatsu K, Arikawa J, Takashima I, Hashimoto N
Laboratory of Public Health, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
Arch Virol. 1998;143(2):365-74. doi: 10.1007/s007050050292.
To understand the mode of transmission of Seoul type hantavirus in Wistar rats, we examined the shedding of the virus and antibody production in infected rats. When 1-day-old rats were inoculated with KI-83-262 strain of Seoul virus, the S segment of the viral genome was detected in lungs, clots, urine, saliva, submaxillary glands, rectums, and kidneys by nested reverse transcriptase PCR. On the other hand, when 8-week-old rats were infected with the virus, viral genome was detected only in the lungs and rectum. In uninfected newborn rats intranasally administered urine from infected newborn rats, four of six rats shed the virus into their urine. In addition, three of eight rats kept in same cage with infected animals also shed the virus into urine. Moreover, the virus genome was detected in the urine of urban rats (Rattus norvegicus) in an enzootic focus. These findings suggest that the urine containing virus from infected rats is an actual source of Seoul virus infection.
为了解汉城型汉坦病毒在Wistar大鼠中的传播方式，我们检测了感染大鼠体内病毒的排出情况及抗体产生情况。当1日龄大鼠接种汉城病毒KI-83-262株时，通过巢式逆转录聚合酶链反应在肺、血凝块、尿液、唾液、颌下腺、直肠和肾脏中检测到病毒基因组的S片段。另一方面，当8周龄大鼠感染该病毒时，仅在肺和直肠中检测到病毒基因组。在未感染的新生大鼠中，经鼻给予感染新生大鼠的尿液，6只大鼠中有4只将病毒排至尿液中。此外，与感染动物同笼饲养的8只大鼠中有3只也将病毒排至尿液中。而且，在一个动物疫源地的城市大鼠（褐家鼠）尿液中检测到病毒基因组。这些发现表明，来自感染大鼠的含病毒尿液是汉城病毒感染的实际来源。
